一株乳酸菌对番茄灰霉病的防效及对几种防御酶活性的影响

摘要

为了研究植物乳杆菌IMAU 10014对番茄的促生长作用和对番茄灰霉病的生防效果以及对植株叶片几种防御酶活性的影响,通过植物乳杆菌IMAU10014发酵液对番茄种子浸种的方法检测促生长作用以及对番茄苗的叶片喷雾和灌根的方法来测定其作为生防杀菌剂的意义.在菌液浓度为107 CFU/mL,对番茄浸种6-8 h,明显提高了种子发芽率及芽长,发芽率达到100％;另外在番茄幼苗接种病原菌后,再由植物乳杆菌IMAU10014做不同处理,病情指数可由47.22分别降至32.41和26.39(防治效果分别达31.36％和44.11％)；与植物防御抗病相关的苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)及多酚氧化酶(PPO)活力都有明显增加.植物乳杆菌IMAU10014作为生物农药防治灰霉病有很大的潜力.%The aim of this study was to investigate Lactobacillus plantarum IMAU10014's growth promotion effects on tomato, biocontrol efficacy on Bottytis cinerea caused disease and its induction effects on several defense-related enzymes. Seed-soaking with IMAU10014 was used for determining its growth promotion effects on tomato, and leaf spraying and root soaking inoculation were employed to determine its biocontrol effectiveness. Seed-soaking with IMAU10014 at concentrations of 107 CFU/mL for 6 to 8 h significantly increased seed germination rates and buds size. Seed germination rate was 100%. Treatment of tomato seedlings with L. Plantarum IMAU10014 reduced B. Cinerea disease index from 47.22 to 32.41 and 26.39 (Biocontrol effect 31.36% and 44.11%), respectively. Significant induction of plant defense-related enzymes, including phenylalanine ammonia-lyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO) was observed in tomato plants treated with L. Plantarum IMAU10014. L. Plantarum IMAU10014 has good potential as a biological pesticide to control Botrytis blight.