大鼠蛛网膜下腔出血后血管平滑肌增殖模型的制备

摘要

Objective The current paper aims to simulate the effects of hemolytic products on intracranial vascular smooth muscle cell after subarachnoid hemorrhage (SAH). and probe into the molecular mechanism and strategy for the prevention and cure of vascular proliferation after SAH. Methods Thirty Sprague-Dawley rats were randomly divided into three groups, including sham-operated. 24 h after SAH, and 72 h after SAH groups. The artificial hemorrhage model around the common carotid artery was established for the latter two groups. The animals were put to death after 24 h and 72 h to take the common carotid artery, and to measure the expression level of PCNA. SM-a-actin protein, and mRNA in the smooth muscle cell. Results The PCNA mRNA expression was significantly up-regulated in the 24-h group (P<0. 01). The expression in the 72-h group was lower than that of the 24-h group (P<0. 01), whereas it was still remarkably higher than that of the sham group (P<0. 01). The SM-oractin mRNA expression in the smooth muscle cell in the 24-h and 72-h groups decreased compared with that of the Sham group (P<0. 05), whereas the 72-h group was significantly lower than that of the 24-h group (P<0. 05). The protein expression of PCNA and SM-oractin showed a similar trend. Conclusion The current experiment simulates better effects of the hemolytic products on vascular smooth muscle cell after SAH. It also shows that artificial hemorrhage around the common carotid artery could stimulate vascular smooth muscle cell to change from contractile phenotype into synthetic phenotype, and improve it to proliferate.%目的 模拟蛛网膜下腔出血 (SAH)后溶血产物对颅内血管平滑肌细胞的影响,探讨SAH后血管增殖的分子机制及防治策略.方法 30只SD大鼠随机分为假手术组(Sham组)、SAH后24h组(24h组)、SAH后72h组(72h组),后两组建立颈总动脉周围人T 出血模型,分别于24h和72h后处死动物取颈总动脉,检测平滑肌细胞中PCNA和SM-α-actin蛋白1及mRNA的表达水平.结果 PCNA mRNA水平在24h组血管平滑肌细胞中表达明显上调(P＜0.01),在72h组中表达较24h组有所减弱(P＜0.01),但仍显著高于Sham组(P＜0.01)；与Sham组比较,24h组和72h组血管平滑肌细胞中SM-α-actin mRNA的表达均明显降低(P＜0.05),且72h组显著低于24h组(P＜0.05).PCNA及SM-α-actin的蛋白表达水平亦显示相似趋势.结论 较好地模拟了SAH后溶血产物对血管平滑肌细胞的影响,显示大鼠颈总动脉周围人工出血能刺激血管平滑肌细胞由收缩表型转化为合成表型,并促进其增殖.