This research was to explore the associations between the MnSOD gene T >C genetic polymorphism with the oxidative stress to exhaustive exercise program among healthy college student .A total of 228 healthy college students were included in the present study .MnSOD mRNA ,enzyme activity ,plasma MDA ,8-iso-PG ,and total glutathione were evaluated before and 1h after an exhaustive exercise .The result indicated that the MnSOD geneT>C was associated with oxidative stress to exhaustive exercise in men and women .People with the MnSOD gene CC genotype had lower MDA and 8-iso-PG values than carriers of the MnSOD gene T allele after the exhaustive training (P< 0 .05) ,and there was a dose response relationship among the three genotype groups (Ptrend< 0 .05) .After the exhaustive training ,the MnSOD mRNA and enzyme activity values were significant in-creased ,and these values in CC genotype were much higher than the T allele .There were also dose response relation-ships among the three genotype groups (Ptrend<0 .05) .It could be seen that the MnSOD gene T>C was associated with oxidative stress to exhaustive exercise by means of affecting the MnSOD gene mRNA expression and enzyme activity .%目的：探讨MnSOD基因T＞C单核苷酸多态性（single nucleotide polymorphism ，SNP）与力竭运动氧化应激的关联。方法：选择228名大学生进行本次研究，测定研究对象最大耗氧量（VO2 max ），在力竭运动过程中测定研究对象VO2 max、呼吸商（respiratory quotient ，RQ）和心率；在力竭运动前后抽取研究对象静脉血，使用酶联免疫吸附法（ELISA ）检测研究对象血浆中丙二醛（malondialdehyde ， MDA）、8-异前列腺素（8-iso-prostaglandin ，8-iso-PG）和总谷胱甘肽（total glutathione ，tGSH）等氧化应激指标，采用实时定量PCR检测MnSOD miRNA的表达量，使用WST 法检测研血浆MnSOD酶的活性；采用PCR-RFLP基因分型技术检测MnSOD基因T＞C位点多态性。结果：MnSOD基因 T＞C单核苷酸多态性与研究对象VO2 max以及力竭运动过程中VO2 max、RQ和心率没有关联，同时与研究对象MDA、8-iso-PG和tGSH指标基础值无关联，但是与力竭运动后MDA和8-iso-PG等氧化应激指标有关联。力竭运动后CC基因型个体M DA和8-iso-PG水平显著低于T C和T T基因型个体，且随着T基因型的增加，MDA 和8-iso-PG 水平也逐渐增加，趋势检验发现具有剂量效应关系（Ptrend＜0．05）。另外功能研究显示，力竭运动后，与 T＞ C位点C基因型相比，T 基因型显著降低MnSOD基因mRNA的表达量以及蛋白酶的活性。结论：MnSOD基因 T＞C单核苷酸多态性与力竭运动氧化应激存在着关联，C基因型个体通过增加MnSOD基因mRNA 的表达量以及蛋白酶的活性，从而降低研究对象力竭运动后机体产生的氧化损伤。
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