Objective To observe the regulatory effect of bFGF and TGF-β1 for the proliferation of mesenchymal progenitor cells ( MPCs) derived from primary osteoarthritis cartilage, and to provide theoretical evidence in preventing and curing primary OA. Methods Different concentrations of bFGF and TGF-β1 ( alone or combined) were used to treat primary OA cartilage and their effects on proliferation of MPCs were tested by MTT method. Results Either bFGF (10. 0~50. 0 ng/mL) or TGF-β1 (0. 1 ~1. 0 ng/mL) alone can significantly promote the proliferation of MPCs derived from primary OA cartilage (P<0. 05). But with their increased concentration,the proliferation rate was of no significant changes (P>0. 05). The combination of 10. 0 ng/mL bFGF and 1. 0 ng/mL TGF-β1 significantly increased the prolifer-ation of MPCs from primary OA (P<0. 05). Conclusion Both bFGF and TGF-β11 play important roles in the proliferation of MPCs in primary OA cartilage,and they can increase the proliferation in different degree with different concentrations. There must be feasible methods of gene technology to promote cell proliferation and differentiation of MPCs for repairing articular car-tilage injury.%目的：观察bFGF和TGF-β1对原发性骨关节炎（ OA)关节软骨间充质祖细胞（ MPCs)的促增殖作用，为通过调控关节软骨MPCs以防治原发性OA提供理论依据。方法采用MTT法测定不同浓度bFGF和TGF-β1单独或联合作用下对原发性OA关节软骨MPCs的增殖作用。结果10.0~50.0 ng/mL的bFGF、0.1~1.0 ng/mL的TGF-β1单独作用于传代培养原发性OA关节软骨MPCs时，其培养细胞的增殖速率显著增加（P<0.05)，而随着二者浓度的进一步增加，其增殖速率无显著变化（P>0.05)；bFGF 10.0 ng/mL+TGF-β11.0 ng/mL联合作用时，对原发性OA关节软骨MPCs具有明显促增殖作用（P<0.05)。结论 bFGF、TGF-β1对原发性OA关节软骨MPCs增殖具有重要作用，不同浓度的bFGF、TGF-β1及bFGF+TGF-β1促增殖作用不同，提示通过适宜浓度的bFGF、TGF-β1对原发性OA关节软骨MPCs的作用，可能具有促进原发性OA关节软骨损伤修复的效果。
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