转录因子T-bet和GATA-3在口腔扁平苔藓患者外周血单核细胞中的表达

摘要

Objective: To investigate the expression of the T cell-specific transcription factors T-bet and GATA-3 in peripheral blood mononuclear cells(PBMCs) of patients with oral lichen planus (OLP).Methods: PBMCs were isolated by density gradient centrifugation from OLP patients with reticular OLP or erythematous/erosive OLP and health controls respectively.Expressions of T-bet and GATA-3 mRNA and protein in the PBMCs were detected by RT-PCR and Western blot respectively.Results: The expressions of T-bet mRNA( relative optical density) in control, reticular OLP and erythematous/erosive OLP groups were 0.57 ± 0.07, 0.34 ± 0.06 and 0.12 ± 0.04 ( P ＜ 0.01 ), the levels of T- bet protein expressions ( photoabserption value) were 0.78 ± 0.07, 0.40 ± 0.05 and 0.11 ±0.02 ( P ＜ 0.01 ), the levels of GATA-3 mRNA expressions were 0.26 ± 0.07, 0.54 ± 0.07 and 0.83 ± 0.09 ( P ＜ 0.0l ), GATA-3 protein expressions were 0.16 ± 0.03, 0.55 ± 0.05 and 0.83 ± 0.06, respectively (P ＜ 0.01 ).Conclusion: Imbalance of transcription factors T-bet and GATA-3 may be one of the key factors in immune dysregulation in patients with OLP.%目的:探讨转录因子T- bet和GATA- 3在口腔扁平苔藓(OLP)患者外周血单核细胞(PBMCs)中的表达及意义.方法:通过密度梯度离心法分离充血糜烂型及光滑型OLP患者和对照组受试者PBMCs,采用RT- PCR和Western blot法分别检测PBMCs中T- bet、GATA- 3 mRNA和蛋白的表达.结果:充血糜烂型、光滑型OLP和对照组外周血单个核细胞中T- bet mRNA表达的相对光密度值分别为0.12±0.04、0.34±0.06和0.57±0.07,T- bet蛋白表达的光吸收度值分别为0.11±0.02、0.40±0.05和0.78±0.07,组间比较差异均有统计学意义(P<0.01);GATA- 3 mRNA表达分别为0.83±0.09、0.54±0.07和0.26±0.07,GATA- 3蛋白表达分别为0.83±0.06、0.55±0.05和0.16±0.03,组间比较差异均有统计学意义(P<0.01).结论:转录因子T- bet/GATA- 3表达失衡是OLP免疫失衡的重要原因之一.