A pot culture experiment was carried out in greenhouse, and proteomics technology was used to study the symbiosis-related proteins between AM fungi and Amorpha fruticosa. Total root proteins of A. fruticosa were extracted by optimized phenol-extracted method, and then the loading amount and staining method were optimized. Results show that high-resolution reproducible two-dimensional electrophoresis (2-DE) gels could be obtained by the optimized phenol-extracted method, using 24 cm IPG strip with a linear gradient of pH 4—7 for IEF, and protein samples loaded with 1 000 mg, visualized by Coomassie brilliant blue R-350. Analyzed with Image Master TM 2D Platinum software ( Version 7. 0) , the 2-DE image of A. fruticosa root proteins inoculated with Glomus mosseae under a colonization rate of up to 90% exhibited 400 protein spots, among which 14 protein spots were up-regulated, 3 protein spots down-regulated, and 15 uniquely expressed.%丛枝菌根(arbuscular mycorrhizal,AM)真菌与宿主植物紫穗槐形成菌根过程中,双方都会发生一系列复杂的形态学和生理学变化,是一个多方面参与并精细调控的信号事件.通过温室盆栽试验,运用蛋白质组技术研究了AM真菌侵染紫穗槐过程中产生的共生相关蛋白,采用改良酚提取法提取紫穗槐菌根蛋白,并对蛋白上样量、染色方法等进行优化.结果表明,采用改良酚提取法,选用24 cm pH值4～7的胶条、1 000 μg上样量、考马斯亮蓝R-350染色可获得分辨率高、重复性好的双向电泳图谱；应用凝胶分析软件Image Master TM 2D Platinum (Version 7.0)分析紫穗槐AM真菌侵染率达到90％时的菌根蛋白,接种AM真菌处理的GM组共获得434个蛋白点,而作为对照的CK组共获得419个蛋白点.其中GM组蛋白点与CK组相比较,上调表达蛋白点14个,下调表达蛋白点3个,特异表达蛋白点15个.
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