诺丽茎段的离体再生

摘要

We established two regeneration systems ( Pattern Ⅰ:adventitious roots were induced after adventitious bud;PatternⅡ:adventitious roots were induced before adventitious bud ) with stem segments as explant in MS culture medium with dif-ferent hormones combinations. The best medium for the inducement of callus was 3%MS+1.0 mg/L 6-BA+0.05 mg/L NAA.In the Pattern Ⅰ, the optimal culture medium for the inducement of adventitious bud from the callus was 3%MS+3.0 mg/L 6-BA, and 3%MS+0.2 mg/L NAA was best for the inducement of adventitious roots .In the PatternⅡ, the op-timal culture medium was 3%MS+0.05 mg/L NAA.%以诺丽（ Morinda citrifolia Linn．）不含腋芽的茎段薄片为外植体，在添加不同种类和质量浓度的3％MS培养基上离体培养，建立模式Ⅰ为先诱导不定芽后诱导不定根、模式Ⅱ为先诱导不定根后诱导不定芽两种离体再生模式。结果表明：诱导诺丽茎薄片产生愈伤组织的最优培养基为3％MS＋1．0 mg/L 6-BA＋0．05 mg/L NAA；在模式Ⅰ中，诱导诺丽茎薄片愈伤组织分化出不定芽的最优培养基为3％MS＋3．0 mg/L 6-BA，诱导不定芽生根的最优培养基为3％MS＋0．2 mg/L NAA；在模式Ⅱ中，诱导诺丽茎薄片的愈伤组织先分化出不定根再分化出不定芽的最优培养基为3％MS＋0．05 mg/L NAA。