激活增强子结合蛋白4在胃癌中的表达及其临床意义

摘要

Objective:To investigate the expression and correlation with clinicopathological parameters of activa-ting enhancer binding protein 4 (AP4)in gastric cancer. Methods:AP4 expression in BGC823 and normal gastric cells was detected by RT-PCR and Western blot. The mRNA and protein expression of AP4 in 90 gastric cancer tis-sues and paired adjacent tissues of 90 cases were investigated by RT-PCR and immunohistochemical SP method. Ka-plan-Meier survival curves,Log-rank test and Cox multivariate test regression were used to analyze the clinicopath-ological prognostic factors of gastric cancer. Results:The mRNA and protein expression of AP4 in gastric cancer cell line (BGC823)was higher than normal gastric cells (P＜0. 05,respectively). AP4 was highly expressed at mRNA levels in the gastric tissue samples compared with the paired adjacent normal tissues (3. 848 ± 0. 426 vs 1. 411 ± 0. 405,P＜0. 05). Furthermore,compared to paired adjacent tissue,AP4 protein was highly expressed in gastric canc-er tissue and the expression level of AP4 protein was significantly associated with vascular invasion (P＜0. 05),depth of invasion (P＜0. 05)and TNM stage (P＜0. 05),but not to gender,age,differential degree,tumor site,lymph node metastasis and distant metastasis (P＞0. 05,respectively). Kaplan-Meier univariate analysis showed that AP4 pro-tein was an important factor for affecting the prognosis of gastric cancer (P＜0. 05),and further Cox regression analy-sis demonstrated that AP4 protein was an independent prognostic risk factor of gastric cancer (P＜0. 05). Conclu-sion:The high expression of AP4 is closely related to the occurrence,development,metastasis and prognosis of gastric cancer,and it may become a new biomarker to assist in the diagnosis and treatment of gastric cancer.%目的:探讨激活增强子结合蛋白4(activating enhancer binding protein 4,AP4)在胃癌组织中的表达及其与临床病理特征的关系.方法:采用RT-PCR和Western blot检测胃癌细胞株BGC823和正常胃上皮细胞中AP4 mRNA和蛋白的表达;应用RT-PCR及免疫组化法检测90例胃癌组织中AP4 mRNA和蛋白的表达;进一步采用Log-rank检验和Cox回归模型对结直肠癌患者的预后因素进行分析,并用Kaplan-Meier计算不同AP4表达情况患者的生存率曲线.结果:与正常胃上皮细胞相比,胃癌细胞株BGC823中AP4 mRNA和蛋白的表达水平均显著升高(均P＜0. 05);在胃癌组织中,AP4 mRNA的表达水平也明显高于配对的癌旁组织(3. 848 ± 0. 426 vs 1. 411 ± 0. 405,P＜0. 05);在蛋白水平,胃癌组织也显著高于配对的癌旁组织,且AP4蛋白表达与胃癌的脉管浸润(P＜0. 05)、浸润深度(P＜0. 05)及TNM分期(P＜0. 05)相关,而与患者的性别、年龄、分化程度、肿瘤位置、淋巴结转移和远处转移无关(均P＞0. 05);Kaplan-Meier单因素回归分析显示,AP4蛋白是影响胃癌患者预后的重要因素(P＜0. 05),进一步Cox多因素回归分析显示AP4蛋白是胃癌患者的独立预后因素(P＜0. 05).结论:AP4高表达与胃癌的发生、发展、转移及预后关系密切,可能成为协助胃癌诊断和治疗的新的生物标志物.