胃癌组织中HPV16与幽门螺杆菌感染相关性研究

摘要

目的:研究胃癌组织中人类乳头瘤病毒16型(HPV 16)感染与幽门螺杆菌感染之间的相关性.方法:运用原位PCR和免疫组化技术分别检测陕西省地区胃癌组织中HPV16 癌基因E6和幽门螺杆菌(Hp).结果:在40例胃癌组织(GC)中HPV16 E6的阳性率为27.5%(11/40) 而在40例癌旁正常组织(GANM)中未检测到;GC组中HPV16 E6 阳性率明显高于GANM组(P=0.0004);贲门癌中HPV16 的感染率明显高于非贲门癌 (P=0.0136);胃癌中HPV16与幽门螺杆菌感染无明显相关性(P=0.0829).HPV16与胃癌患者的性别、年龄、肿瘤浸润、淋巴结转移以及病理分级均无明显相关性(P>0.05).结论:本研究结果提示,在胃癌的发生过程中,HPV16可能不依赖或者不与幽门螺杆菌协作而发挥作用.%Objective:To investigate the relationship between HPV16 and Helicobacter pylori (Hp) infection in human gastric carcinoma(GC). Methods: in situ PCR (ISPCR) method was carried out to detect HPV16 oncogene E6 in 40 GCs and corresponding gastric adjacent normal musoca (GANM) from Shaanxi Province of China.Helicobacter pylori (Hp) was detected by immunohistochemistry(IHC) in gastric tissues. Results: The positive rates of E6 were 27.5%(11/40) in GCs and 0%(0/40) in GANMs respectively with ISPCR.HPV16 DNA was mainly located in the nucleus of gastric glandular epithelium cells.The infection rate of HPV16 DNA in GCs was significantly higher than that in GANMs (P=0.0004),and the HPV16 had no statistical correlations with gender,age,invasion,grading and lymph node metastasis respectively (P>0.05). The infection rate of HPV16 in cardiac GCs was significantlyhigher than that in non-cardiac ones(P=0.0136),and HPV16 had no correlation with Hp in GCs (P=0.0829). Conclusions: These findings suggested that HPV16 can infect gastric glandular epithelium cells and that viral infection might play a role in the occurrence of GCs independently or noncooperatively with Hp infection.