Objective :To assess the effect of paclitaxel on TRAIL - induced apoptosis in gastric: cancer cells, and the action of death receptor 5 ( DR5 ) in TRAIL - induced apoptosis. Methods : Cell proliferation was measured using MTT assay. Cell apoptosis was determined by flow cytometry. Expression of proteins was analyzed by western blot. Results : Treatment with 100 ng/ml TRAIL for 24 h resulted in a slight reduction in cell viability and a little increase in cell apoptosis in MGC803 cells. Treatment with TRAIL ( 100 ng/ml ) and paclitaxel (3. 41 g/ml ) leaded to a significant reduction in cell viability and a dramatic increase in cell apoptosis ( P ＜ 0. 05 ). TRAIL alone did not change the expression of death receptor 5 ( DR5 ) , while 3. 41 g/ml paclitaxel significantly upregulated the expression of DR5 in MGC803 cells. Conclusion: Paclitaxel enhanced TRAIL - induced apoptosis in gastric cancer MGC803 cells by upregulating the expression of DR5 .%目的:研究紫杉醇对TRAIL诱导胃癌细胞凋亡的影响,探讨死亡受体5(DR5)在TRAIL诱导细胞凋亡中的作用.方法:采用MTT法测定细胞活力,流式细胞仪检测细胞凋亡,western blot检测蛋白表达.结果:在MGC803细胞中,100ng/ml的TRAIL 导致轻度的增殖抑制和细胞凋亡,TRAIL(100ng/ml) 联合紫杉醇(3.41g/ml)引起明显的增殖抑制和细胞凋亡(P<0.05).TRAIL单药没有改变死亡受体5(DR5)的表达,而3.41g/ml紫杉醇作用MGC803细胞后明显上调了DR5的表达.结论:紫杉醇通过上调DR5的表达增强TRAIL诱导的胃癌MGC803细胞凋亡.
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