MACC1基因siRNA对肺癌SBC-5细胞增殖和迁移的影响

摘要

目的:探讨MACC1(metastasis-associated in colon cancer l)基因特异性siRNA(small interfering RNA)对肺癌细胞系SBC-5体外增殖和迁移的影响.方法:化学合成MACC1基因特异性siRNA,阳离子脂质体LipofectamineTM 2000转染肺癌细胞系SBC-5细胞,RT-PCR和Western blot方法检测转染后SBC-5细胞中MACC1基因和蛋白的表达情况;MTT法检测细胞的生长增殖状况;划痕试验观察细胞迁移能力的改变.结果:MACC1基因特异性siRNA转染细胞后,SBC-5细胞MACC1的mRNA和蛋白表达水平明显降低,MTT试验和划痕试验观察到转染MACC1-siRNA的SBC-5细胞增殖、迁移能力明显减弱.结论:MACC1基因特异性siRNA可以明显抑制肺癌SBC-5细胞增殖和迁移,MACC1可能成为肺癌治疗的新靶点.%Objective: To investigate the influence of siRNA targeting metastasis - associated in colon cancer Ⅰ ( MACC1 ) gene on proliferation and migration of human lung cancer cell line SBC - 5 in vitro. Methods : The siRNA targeting MACC1 gene and negative control siRNA were chemically synthesized, and were then transiently transfected into human lung cancer cell line SBC - 5 hy LipofectamineTM 2000. Expression of MACC1 mRNA and protein in siRNA transfected cells were examined by RT - PCR and Western blotting analysis. Proliferation and migration of siRNA transfected SBC - 5 cells were examined by MTT and Wound healing assay. Results : Transfection of specific siRNA targeting MACC1 into SBC - 5 cells inhihited MACC1 gene expression and both mRNA and protein expression levels declined markedly. siRNA transfected SBC - 5 cells showed decreased ability of proliferation and migration. Conclusion : The specific siRNA targeting MACC1 gene can significantly inhibit proliferation and migration of SBC - 5 cells, MACC1 may serve as a potential target for gene therapy of lung cancer.