Objective;To study the level of 99mTc - HL91 hypoxia imaging in esophageal cancer and correlation between 99mTc - HL91 uptake and expression of HIF - 1α.Methods: Fifty patients with esophageal cancer underwent 99mTc - HL91 hypoxia imaging.Regions of interest( ROI) were drawn on the tumor( T) and contralateral normal esophageal tissue( N) , T/N ratios were measured for all scans.The expression of HIF - 1α was detected by immunohistochemical method.Results :In all the 50 patlents, 99mTc - HL91 uptake of tumor tissue was obviously higher than that of normal tissue , and no relevance to the tumor pathologic classification ( P ＞ 0.05 ) .Four hours after injecting of 99mTc - HL91 , the T/N of cervical and upper chest , middle chest and inferior chest esophageal cancer were 2.04 ± 0.38 ,2.16 ± 0.32,2.18 ± 0.41 respectively( P ＞ 0.05 ) .In all the 50 patients , T/N ratio ranged from 1.36 -4.43 ( median : 2.45) , the percentage of tumor cells expressing; HIF - 1α was 23.1％ - 76.9％ ( median :52.9％ ) , and a positive correlation was found between them ( P＜ 0.05 ) .Conclusion : The tissue of esophageal cancer can uptake 99mTc - HL91 obviously and haa no relevance to pathologic classification and lesion pcniition but with a positive correlation with HIF - 1α.%目的:研究食管癌99mTc- HL91 乏氧显像以及与乏氧诱导因子-1α(HIF-1α) 表达水平的相关性.方法:50 例食管癌患者行SPECT 99mTc- HL91 乏氧显像, 利用感兴趣区技术分别勾画各时相肿瘤(T) 和相应正常食管部位(N) 放射性计数比值,即T/N值, 免疫组化方法检测肿瘤组织HIF-1α表达水平.结果:50例病灶对99mTc- HL91 的摄取均高于相应正常组织, 99mTc- HL91 摄取程度与肿瘤病理分级无相关关系( P>0.05) ; 注射99mTc- HL91 4小时后SPECT 显像颈段和胸上段食管癌T/N 值为2.04±0.38, 胸中段食管癌T/N 值为2.16±0.32, 胸下段食管癌T/N 值为2.18±0.41,三者无显著性差异( P>0.05) ; 病灶的T/N 值为1.36-4.43( 中位值2.45) , 免疫组化结果显示表达HIF- 1α的细胞数为23.1%-76.9%( 中位数52.9%) , 两者之间呈正相关( P＜0.05) .结论:食管癌组织对乏氧显像剂99mTc- HL91 有显著摄取, 摄取值与肿瘤病理分级及病变部位无相关性, 但与HIF- 1α的表达正相关.
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