ADAM-9 在肝细胞肝癌中的表达及意义

摘要

Objective:To investigate the clinical significance of ADAM -9 in hepatocellular carcinoma (HCC). Methods: One hundred and sixty - seven cases of HCC were tested for expression of ADAM — 9 by SP immunohisto-chemistry. Results: Among 167 HCC samples,49 (29%) were negative for ADAM -9,118 (71%) had positive ADAM - 9 immunoreactivity. The ADAM - 9 - positive cells in HCC tissues showed unequivocal cytoplasmic and membrane cellular staining pattern. Among 56 normal liver tissue samples,38% of them were positive for ADAM - 9. Significant difference was found between HCC group and normal liver tissue group. HCC tissues with positive ADAM - 9 expression were larger and less differentiated than those with negative expression (P < 0. 05 ). Portal venous invasion , hepatic venous invasion, bile duct invasion, intrahepatic metastasis and high AFP levels were detected significantly more frequently in ADAM — 9 positive group ( P < 0. 05 ). Moreover, ADAM — 9 positive group had significantly poorer outcomes than ADAM - 9 negative group (P < 0. 05 ) . COX analysis showel that AMAD - 9 was an independent prognostic factor for overall survival (P < 0. 05 ). Conclusion: ADAM - 9 may play an important role in the development and metastasis of HCC.%目的:研究ADAM-9在肝细胞肝癌(HCC)中的表达及其与临床病理参数、预后之间的关系.方法:利用免疫组化方法检测167例HCC组织中ADAM-9的表达情况.结果:167例HCC标本中,49例(29％)标本为ADAM-9阴性,118例(71％)标本为ADAM-9阳性；而在56例正常肝组织中间质ADAM-9的表达阳性率为38％,两者比较有显著差异(P＜0.05).HCC组织ADAM-9阳性细胞表现出典型的细胞膜和细胞质型表达.单因素分析结果显示ADAM-9在HCC癌组织的表达与肿瘤的大小、分化程度、侵袭转移、高AFP水平和复发有关,差异均具有统计学意义(P＜0.05)；K-M生存曲线显示ADAM-9阴性表达组生存率高于ADAM-9阳性表达组,差异具有统计学意义(P＜0.05).多因素COX逐步回归分析结果显示ADAM-9是总体生存率的独立因素(P＜0.05).结论:ADAM-9在HCC中的过表达,对HCC的发生、侵袭转移及预后起重要作用.