目的:探讨环氧化酶2(COX -2)抑制剂塞来昔布对人胃癌BGc823细胞株的放射增敏作用及其机制.方法:MTT实验测定塞来昔布对胃癌BGC823细胞株的抑制率,计算半数抑制浓度(IC50).克隆形成实验检测塞来昔布联合不同剂量X线照射后细胞的存活率,计算放射敏感性相关参数,评价增敏效果.流式细胞仪(FcM)分析细胞周期分布情况.结果:MTT实验显示塞来昔布对BGC823细胞作用24h、48h、72h的IC50分别是162.6 μmol/L、95.4μmol/L、62.1μmol/L；克隆形成实验测得联合组的Do、Dq及SF2均明显低于单纯照射组(1.705VS 2.185,1.032VS 2.327,0.495 VS 0.745),SER为1.3.流式细胞仪检测联合组Go/G1期细胞增多,S期细胞减少.结论:塞来昔布能增强胃癌BGC823细胞的放射敏感性,其机制可能与减少肿瘤细胞亚致死性损伤修复和细胞周期再分布有关.%Objective: To test the effect of celecoxib, alone or combined with radiation, on human gastric carcinoma cell line BGC823 and investigate the potential mechanism in vitro. Methods: Human gastric carcinoma cell line BGC823 was tested by MTT assay for cell proliferation, colony - forming assay for cell radiosensitivity, flow cytometry assay for cell cycle distribution. Results: The IC50 of celecoxib treated with BCC823 cells for 24h was 162. 6μmol/ L,for 48h was 95.4μmol/L, and for 72h was 62. 1 μmol/L. The D0 , Dq and SF2 values for irradiated BGC823 cells pretreated with celecoxib were lower than those for unpretreated ones (1.705 VS 2.185,1.032 VS 2.327,0.495 VS 0.745). The sensitization enhancement (SER) ratio was 1.3. The result of the cell cycles showed that BGC82 cells arrested in C0/G1 phase in the combination group. Conclusion; Celecoxib enhanced radiosensitization on human gastric carcinoma BGC823 cells. The mechanism may be involved with repair inhibition of sublethal damage and redistribution of cell cycle.
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