Objective:To investigate the effect of IFN - α on TRAIL - induced apoptosis of gastric cancer and e-valuate the role of death receptor DR5 and PI3K/Akt pathway during the process. Methods: Cell proliferation was measured by MTT assay and cell apoptosis was determined by flow cytometry using propidium iodide staining. Protein expression was assayed by Western blot. Results: In gastric cancer MGC803 cells, preincubation with IFN - α enhanced the TRAIL - induced cytotoxicity. Only slight cell apoptosis was induced by IFN - α alone and TRAIL alone. In contrast, pretreatment of cells with IFN - α significantly amplified TRAIL - induced cell apoptosis. IFN - α up-regulated DR5 expression and inhibited the activity of Ak. Conclusion: IFN - α enhanced TRAIL - induced apoptosis in gastric cancer cells might be correlated with upregulation of DR5 and inhibition of PDK/Akt pathway.%目的:观察IFN -α对TRAIL诱导胃癌细胞凋亡的影响,并对死亡受体DR5和PI3 K/A kt通路在此过程中的作用机制进行探讨.方法:MTT法检测细胞增殖能力,流式细胞仪PI染色检测细胞凋亡,蛋白质印迹法检测蛋白的表达.结果:IFN-α预处理可协同TRAIL抑制胃癌MGC803细胞增殖.单独用IFN -α或TRAIL处理MGC803细胞,仅有少量的细胞发生凋亡,IFN -α和TRAIL联合用药细胞凋亡明显增加(P＜0.01).IFN -α能上调DR5的表达,抑制Akt的磷酸化.结论:IFN -α能增强TRAIL诱导的胃癌细胞凋亡,其机制可能与其上调DR5的表达和抑制Akt的活性有关.
展开▼
