散发性乳腺癌中MTA1表达与ERα甲基化关系的研究

摘要

目的:通过甲基化特异性PCR和免疫组化研究女性散发性乳腺癌中ERα基因启动子区甲基化和MTA1蛋白表达的相关性.方法:甲基化PCR研究102例散发性乳腺癌ERα启动子甲基化情况,免疫组化研究其MTA1蛋白表达情况.结果:乳腺癌组织中ERα启动子甲基化率为37.3％,乳腺癌组织中MTA1表达率为29.4％,高于其在癌旁正常乳腺组织中的表达(P＜0.05),MTA1表达和ERα启动子甲基化均与乳腺肿瘤大小、TNM分期及淋巴结转移相关(P＜0.05),且乳腺癌中ERα启动子甲基化与MTA1阳性表达呈正相关(P＜0.05).结论:女性散发性乳腺癌中ERα基因启动子甲基化与MTA1表达升高密切相关,其在乳腺癌发展过程中可能起重要作用.%Objective:To study the relation between the promoter methylation of estrogen receptor-alpha and MTA1 expression in sporadic breast cancer by methylation-specific polymerase chain reaction and immunohistochemistry.Methods:The methylation of ERα promoter was investigated by methylation-specific polymerase chain reaction and MTA1 expression was analyzed by immunohistochemistry in 102 sporadic breast cancer.Results:ERα methylation was observed in 37.3％ breast cancer tissues and MTA1 expression in breast cancer (30/102,29.4％)was higher than that in non-neoplastic breast tissues (6/102,5.9％) (P ＜ 0.05).Both of them were significantly associated with lymph node metastasis and tumor size and TNM stage in breast cancer (P ＜ 0.05).ERα methylation had significant correlation with the MTA1 expression (P ＜ 0.05).Conclusion:The ERα methylation status had tight relation with MTA1 expression in female sporadic breast cancer,and they may play important roles in the carcinogenesis of breast cancer.