槲皮素对体外培养宫颈癌细胞生物学行为的影响

摘要

Objective:To study the effects of Quercetin on biological behaviour of uterine cervical carcinoma cell line eela in vitro and its mechanism. Methods:The growth and Proliferation of eela cell was detected by MTT assay and Clone formation test. Cell aPoPtosis and cell cycle were detected by the flow cytometry. Cell invasive ability was measured with Transwell assay. The exPression of Bcl - 2 Protein was detected by Western blot. Results:Quercetin could inhibit cell Proliferation,induce cell aPoPtosis,arrest cell cycle in G2 / M Phase and decrease invasive ability of eela cells in a dose - dePendent manner and the level of Bcl - 2 Protein was markedly suPPressed by Quercetin. Con-clusion:Quercetin suPPressed the Proliferation,induced aPoPtosis and decreased invasion in eela cells by down - reg-ulating Bcl - 2.%目的：研究槲皮素对体外培养宫颈癌细胞增殖、凋亡和转移的影响及其分子机制。方法：采用 MTT法和细胞克隆形成法检测槲皮素对宫颈癌细胞增殖的影响；流式细胞法检测细胞凋亡及周期分布；Transwell法检测细胞体外侵袭力的改变；免疫印迹法检测 Bcl -2蛋白表达水平。结果：经不同浓度槲皮素处理后，宫颈癌细胞的增殖和侵袭能力明显受到抑制，凋亡率显著增高，呈量效关系。随着槲皮素浓度升高，Bcl -2蛋白表达水平明显下降。结论：槲皮素可对宫颈癌细胞产生抑制增殖、诱导凋亡、降低侵袭和转移等作用，可能与其下调细胞内 Bcl -2蛋白表达有关。