淋巴管密度和基质金属蛋白酶表达在结直肠癌中的临床意义

摘要

Objective:To determine the clinical significances of lymPhatic microvessel density(LVD)and matrix metalloProteinase - 1(MMP - 1)in colorectal carcinoma and the correlation between the two indicators. Methods:The LVD stained by D2 - 40 and MMP - 1 exPression in cancerous tissue and normal tissue surrounding carcinoma of 76 Patients with colorectal carcinoma were measured using strePtavidin - biotin technology and the relevances of the both indicators to clinical manifestations were evaluated. Moreover,the correlation between LVD stained by D2 - 40 and MMP - 1 exPression was Performed by Pearson analysis. Results:The LVD stained by D2 - 40 in cancerous tissue was significantly higher than that of normal tissue surrounding carcinoma. The LVD was closely related to dePth of in-vasion,tumor size,lymPh node metastasis and Dukes classification. The Positive incidence of MMP - 1 exPression in cancerous tissue was significantly higher than that of normal tissue surrounding carcinoma. The integral for MMP - 1 exPression was closely related to dePth of invasion,lymPh node metastasis and Dukes classification. The Pearson corre-lation analysis indicated that the integral for MMP - 1 exPression was significantly Positively correlated with LVD stained by D2 - 40. Coclusion:The D2 - 40 LVD and MMP - 1 exPression can be used as one of the imPortant Prog-nostic Parameters for colorectal cancer Patients.%目的：探讨结直肠癌中 D2-40标记淋巴管密度（lymPhatic microvessel density，LVD）和基质金属蛋白酶-1（matrix metalloProteinase -1，MMP -1）表达与肿瘤病理参数的关系，以及二者之间的相关性。方法：采用免疫组织化学链霉亲和素-生物素技术检测76例结直肠癌患者癌组织和癌旁正常组织中 D2-40标记LVD 和 MMP -1表达，分析其与肿瘤临床病理参数的关系，并行结直肠癌组织 MMP -1积分与 D2-40标记LVD 的 Pearson 相关性分析。结果：结直肠癌组织中 D2-40标记 LVD 显著高于癌旁正常组织。LVD 与肿瘤的浸润深度、大小、淋巴结转移和 Dukes 分期相关；结直肠癌组织中 MMP -1阳性表达率较癌旁正常组织显著增高。MMP -1表达与肿瘤的浸润深度、淋巴结转移和 Dukes 分期密切相关。Pearson 相关分析表明，结直肠癌组织中 MMP -1积分与 D2-40标记 LVD 具有显著正相关性。结论：D2-40标记 LVD 和 MMP -1表达可作为结直肠癌患者判断预后的指标。