NRF2/HO-1因子与结直肠肿瘤TNM分期的相关性研究

摘要

Objective:To study the expression of nuclear factor-erythroid2(NRF2),heme oxygenase-1(HO-1) in colorectal tissue,metastatic lymph nodes and metastatic liver tumors. Explore its function in colorectal carcinoma de-velopment and metastasis. Methods:The expression of NRF2/HO-1 in 84 colorectal carcinoma,6 polyps and 10 nor-mal liver tissue paraffin was detected by immunohistochemistry method and the mean optical density of positive cells measured by image analysis technology. Results:According to the mean optical density of NRF2/HO-1 in colorectal carcinoma,the pearson of the scatterplot was 0. 876. It means two factors significantly associated. The mean optical density of NRF2/HO-1 was higher in colorectal carcinoma than negative control group,there was statistical signifi-cance(P<0. 05). The mean optical density of NRF2/HO-1 increased in colorectal carcinoma with the stages of clini-cal cases and the degree of carcinoma differentiation rise(P<0. 05). The mean optical density of NRF2/HO-1 in-creased in metastatic lymph nodes and colorectal carcinoma according to N stage(N1-N2)(P<0. 05). The mean opti-cal density of NRF2/HO-1 was higher in metastatic liver tumors than normol liver (P<0. 05). The mean optical den-sity of NRF2/HO-1 had no correlation with age,gender and T stage. Conclusion:The mean optical density of NRF2/HO-1 was highly expressed in colorectal carcinoma tissue metastatic lymph nodes and metastatic liver tumors. It is probably related with occurrence,development and prognosis of colorectal carcinoma,witch provides an important basis for early prevention,early diagnosis of colorectal carcinoma and selection of treatment.%目的：：检测NRF2、血红素氧合酶-1(heme oxygenase,HO-1)在结直肠癌、转移淋巴结及肝脏转移灶中的表达情况,探讨它们在结直肠肿瘤发生发展及转移( TNM分期)中的作用。方法：采用免疫组化的方法检测84例结直肠癌、6例结直肠炎性息肉、10例正常肝脏组织的石蜡标本中的NRF2、HO-1因子表达情况,使用图像分析系统测定阳性细胞平均光密度值。结果：根据NRF2/HO-1因子在肠癌中的表达情况得出散点图的Pearson 相关性=0.876,提示两因子显著相关；结直肠癌组织NRF2/HO-1的平均光密度值高于阴性对照组,差异有统计学意义(P<0.05)；结直肠癌组织NRF2/HO-1的平均光密度值随着临床病理分期程度和病理分化程度的提高而升高(P<0.05)；在结直肠组织及转移淋巴结中NRF2/HO-1的平均光密度值都按照N分期(N1-N2)逐渐递增(P<0.05)；在肝脏转移灶中NRF2/HO-1的平均光密度值高于正常肝脏组织(P<0.05)；NRF2/HO-1的平均光密度值与年龄、性别、T分期无关。结论：NRF2/HO-1在结直肠癌、转移淋巴结及肝脏转移灶中呈现高表达,可能与结直肠恶性肿瘤发生发展存在关系,是判断结直肠癌的发生、发展及预后的有效指标。