目的：研究鱼藤酮致帕金森病（ Parkinsonˊs disease，PD）大鼠脑黑质中凋亡相关蛋白Smac和Bcl-2表达的改变。方法：将Witstar大鼠随机分为对照组和实验组。对照组10只背部皮下注射葵花油1ml/kg，实验组25只分为A、B、C三组，按照3.0（5只）、2.0（10只）和1.0（10只）mg/（kg·d）背部皮下注射鱼藤酮（鱼藤酮溶解在葵花籽油中，充分震荡混匀后4℃避光保存）。结果：透射电镜观察下，鱼藤酮处置的实验组神经元细胞皱缩，胞质致密，核染色质边集，有部分细胞胞核裂解，胞质芽突脱落，形成凋亡小体。并且随着鱼藤酮染毒剂量的增大，凋亡小体形成更加明显。免疫组化染色显示，Smac的阳性表达实验组高于对照组，Bcl-2的阳性表达实验组低于对照组。结论：鱼藤酮具有明显的神经毒性，能导致大鼠脑内DA能神经元的损伤，细胞凋亡参与了鱼藤酮帕金森模型大鼠黑质多巴胺神经细胞的损伤。%Objective:To study the changes of expression of apoptosis-related proteins Smac and Bcl-2 in the midbrain substantia nigra in Parkinsonˊs disease rat induced by Rotenone. Methods:Witstar rats were randomly divid-ed into control group and experimental group. Control group 10 rats were treated by subcutaneously injection of sun-flower oil 1ml/kg,25 in experimental group were divided into A,B,C three groups,with 3. 0(5),2. 0(10)and 1. 0 (10)mg/(kg·d)(subcutaneous injection of Rotenone dissolved in sunflower oil,shake evenly mixed 4℃ stored a-way from light). Results:TEM observated results,neuronal cells in experimental group shrinkage Rotenone treatment, cytoplasmic dense,the nuclear chromatin,nuclear fragmentation and some cells,cytoplasmic buds abscission,and for-mation of apoptotic bodies. And with the increase of the dose of Rotenone,apoptotic body formation was more obvious.Immunohistochemical staining showed positive expression of Smac,the experimental group was higher than that in the control group,the positive expression of Bcl-2 in experimental group was lower than control group. Conclusion:The results of this study confirmed the neurotoxicity of Rotenone can cause the rat DA ergic neurons in brain injury,apop-tosis participates in Rotenone Parkinson model rat nigral dopaminergic nerve cell damage.
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