Raf 激酶抑制蛋白启动子甲基化与肺癌患者预后的关系

摘要

Objective:Observing the expression of Raf kinase inhibitor protein and its promoter methylation status in lung cancer,to research the relationship between the promoter methylation of Raf kinase inhibitor protein and the prognosis of lung cancer patients. Methods:Using immunohistochemistry(SABC)method detected the expression of Raf kinase inhibitor protein in tissues,using methylation specific PCR method(MSP)detected the promoter methyla-tion of Raf kinase inhibitor protein(RKIP). Using SPSS 15. 0 statistical software to analyze the difference of Raf ki-nase inhibitor protein promoter methylation and the correlation with the prognosis of lung cancer patients. Results:Raf kinase inhibitor protein expression in lung cancer tissues was significantly lower than that in normal tissues adjacent to cancer. Raf kinase inhibitor protein promoter methylation in lung cancer tissues occurred methylation or partial methyl-ation,significantly higher than the normal tissue adjacent to cancer. Raf kinase inhibitor protein promoter methylation was associated with the prognosis of lung cancer patients. Conclusion:Raf kinase inhibitor protein in lung cancer tis-sues was low expressed,one of the important reasons is its promoter methylation,and Raf kinase inhibitor protein pro-moter methylation is an independent predictor of prognosis in lung cancer patients.%目的：观察 Raf 激酶抑制蛋白在肺癌中的表达及其启动子甲基化的情况，研究 Raf 激酶抑制蛋白启动子甲基化与肺癌患者预后的关系。方法：运用免疫组织化学（SABC）方法检测 Raf 激酶抑制蛋白在组织中的表达情况，采用甲基化特异性 PCR 法检测 Raf 激酶抑制蛋白启动子甲基化的情况，SPSS 15.0统计分析 Raf激酶抑制蛋白表达和启动子甲基化的差异及其与肺癌患者预后的相关性。结果：Raf 激酶抑制蛋白在肺癌组织的表达明显低于癌旁组织；Raf 激酶抑制蛋白启动子甲基化在肺癌组织中发生甲基化或部分甲基化，明显高于癌旁组织；Raf 激酶抑制蛋白启动子甲基化与肺癌患者的预后负相关。结论：肺癌患者预后差的一个重要原因是 Raf 激酶抑制蛋白表达低，其机制主要是其启动子发生了甲基化。因此 Raf 激酶抑制蛋白启动子甲基化是预测肺癌患者预后的一个独立因素。