调气消积汤对人肺腺癌 A549细胞肿瘤相关基因表达的影响

摘要

Objective:To approach the molecular mechanism of Tiaoqixiaoji Ddecoction(TQXJ)on tumor -asso-ciated genes expression of IL -8,S28,Sp1 of human lung adenocarcinoma A549 cells.Methods:Cells were divided into 5 groups:Blank group,TQXJ low,median,high dose groups(concentrations were 5%,15%,20%)and DDP group,regular prepare concentrations of drug serum.A549 cells after treatment with different concentrations of Tiao-qixiaoji Decoction serum were observed.The flow cytometry was used to determine the cell cycle,and the genes ex-pression of IL -8,S28,Sp1 mRNA were detected by RT -PCR method.Results:With the increase of drug concentra-tion,the proportion of cells in G0 /G1 phase was decreased,and that in S phase was increased for the each dose TQXJ groups than that of the blank group(P <0.01).Each dose TQXJ group and DDP group all could reduce the expres-sion of IL -8,S28,Sp 1 mRNA,there were statistical difference compared with the blank group (P <0.05 or P <0.01).Compared with high dose TQXJ group and the DDP group,there was no statistical difference between them(P>0.05).Conclusion:The TQXJ may block the cancer cells entering the proliferative phase,and it's activity of inhibi-ting the growth of tumor may be relative to decreasing the expression of IL -8,S28,Sp1 mRNA.%目的：检测调气消积汤对人肺腺癌 A549细胞周期及肿瘤相关基因 IL －8、S28、Sp1表达的影响，探讨该方抗肺癌的分子机制。方法：实验共分为5组：空白对照组、调气消积汤低、中、高剂量组（浓度分别为5％、15％、20％）及顺铂组，常规方法制备含药血清。以不同剂量的调气消积汤及顺铂制作含药血清并分别作用于 A549细胞，用流式细胞仪检测细胞周期，采用 RT －PCR 法检测各组 IL －8、S28、Sp1 mRNA 的表达水平。结果：随着药物浓度的增加，调气消积汤各剂量组的 G0／G1期细胞比例逐渐降低，S 期细胞比例逐渐升高（P ＜0．01）。调气消积汤各剂量组及顺铂组 IL －8、S28、Sp1 mRNA 表达水平明显低于空白对照组，差异有统计学意义（P ＜0．05或 P ＜0．01）。调气消积汤高剂量组与顺铂组比较，差异无统计学意义（P ＞0．05）。结论：调气消积汤可以阻止肿瘤细胞进入增殖周期；下调相关基因 IL －8、S28、Sp1 mRNA 的表达水平，可能是调气消积汤发挥抑瘤作用的分子机制之一。