目的:探讨调气消积汤对Lewis肺癌细胞凋亡及p53、Bcl-2蛋白表达的影响.方法:C57BL/6小鼠常规接种Lewis肺癌,随机分为荷瘤对照组(MG)、调气消积汤组(TG)、顺铂组(DG)、综合组(ZG),每组10只.每天分别予饮用水、调气消积汤各0.4ml灌胃12天;隔日一次腹腔注射生理盐水、顺铂0.13ml共3天.应用电镜技术检测调气消积汤对Lewis肺癌细胞凋亡的影响,用Western Blot法检测p53、Bcl-2基因蛋白的表达.结果:电镜下可见细胞凋亡的特异性标志-凋亡小体形成.调气消积汤组、顺铂组及综合组均可降低p53、Bcl-2蛋白的表达,与荷瘤对照组相比,差异有统计学意义(P<0.05 或 P<0.01).结论:调气消积汤能促进Lewis肺癌小鼠肿瘤细胞凋亡,与化疗药物合用能增强促肺癌细胞凋亡的能力;下调凋亡相关基因突变型p53、Bcl-2的表达水平,可能是调气消积汤发挥抑瘤作用的分子机制之一.%Objective:To study the therapeutic: effect of Tiaoqixiaoji Decoction( TQXJ ) on apoptosis and the expression of p53、Bcl - 2 of mice bearing Lewis lung cancer cells. Methods: Inoculated C57BL/6 mice with Lewis lung cancer cells , then randomly divided into : Model group( MG ),TQXJ group( BG ) ,DDP ( cisplatin ) group ( DG ) and TQXJ - DDP combination group( ZG ),10 mice in each group. Different drug were given to four groups. Determination of cell apoptosis of tumor tissue were performed by electron microscope ( EM ) ; The expression of p53 , Bcl -2 of tumor tissues were determined by Western Blotting method. Results: Through the observation of EM, we found the specific signs of apoptosis - apoptotic bodies. Tiaoqixiaoji Decoction and combined DDP could enhance the ability to promote apoptosis in lung cancer cells. The TG,DG and ZG all could reduce the expression of p53 ,Bcl - 2 of tumor tissue, there were statistical differences compared with the MG( P ＜0. 05 or P ＜ 0. 01 ). Conclusion: Tiaoqixiaoji Decoction can induce the apoptosis of Lewis lung cancer cells, if combined with DDP, it can enhance the ability to promote apoptosis; its activity of inhibiting the growth of tumor may he relative to decreasing the expression of p53 and Bcl -2.
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