雷帕霉素单独及联合化疗药物应用对胃癌细胞生长的影响

摘要

Objective:To investigate the influence of rapamycin on human gastric cancer SGC -7901 cells prolif-eration and its mechanism.Methods:Gastric cancer cells SGC -7901 were cultured and treated with rapamycin,vin-cristine and combination of the two.Then the inhibitory effects of the three medications on the growth of the SGC -7901 cells were determined by MTT,and their cell cycle were detected by flow cytometry.Results:Compared with the control group,the three medications all significantly inhibited the proliferation of SGC -7901 cells,and the combined method enhanced the effect.Flow cytometry showed that rapamycin and vincristine blocked the cell cycle mainly in the G0 /G1 stage,the combined medication enhanced this effect.Conclusion:Rapamycin can inhibit the proliferation of SGC -7901 cells and enhance the chemosensitivity of gastric cancer.%目的：研究 mTOR 抑制剂雷帕霉素对 SGC －7901胃癌细胞增殖的影响及其可能的机制，并探讨其与传统化疗药物的联合作用。方法：体外培养胃癌 SGC －7901细胞，取对数生长期的细胞进行试验，用不同浓度的 mTOR 抑制剂雷帕霉素以及长春新碱分别作用于胃癌 SGC －7901细胞24、48、72小时，用 MTT 法检测雷帕霉素单独以及联合化疗药物对其生长的影响。用流式细胞术检测 mTOR 抑制剂雷帕霉素、长春新碱单独以及联合应用对胃癌 SGC －7901细胞周期的影响。结果：MTT 法结果显示：雷帕霉素单独能够显著抑制胃癌SGC －7901细胞的增殖，其作用随着药物浓度以及作用时间的增加而增加，呈现时间－剂量效应关系；与化疗药物长春新碱联合应用其抑制效应明显增强。流式细胞术检测结果显示：雷帕霉素、长春新碱单独作用胃癌细胞将细胞周期主要阻滞在 G0／G1期，而联合给药时 G0／G1期比例增加。结论：mTOR 抑制剂雷帕霉素能够显著抑制胃癌 SGC －7901细胞的增殖，其机制可能与通过阻滞细胞周期在 G0／G1期相关；并且雷帕霉素可提高传统化疗药物长春新碱对胃癌细胞的敏感性。