miR－300对人卵巢癌细胞增殖、周期及凋亡的影响

摘要

目的：研究 miR－300对人卵巢癌细胞增殖、凋亡和周期的影响。方法：通过荧光实时定量 PCR （qRT－PCR）检测卵巢正常上皮细胞及卵巢癌细胞中miR－300的表达水平和miR－300 siRNA的沉默效果；CCK－8法检测沉默miR－300对卵巢癌细胞增殖的影响；流式细胞术检测沉默miR－300对卵巢癌细胞凋亡和周期的影响。结果：miR－300在卵巢癌细胞中的表达显著高于卵巢正常上皮细胞；miR－300 siRNA可有效沉默卵巢癌细胞中miR－300的表达；沉默miR－300的表达可以明显抑制卵巢癌细胞的增殖能力，增强卵巢癌细胞的凋亡能力，并使卵巢癌细胞的周期阻滞于G1期。结论：miR－300在卵巢癌细胞的增殖、凋亡和周期过程中起着重要的调控作用。%Objective:To investigate the effects of miR-300 on the proliferation,apoptosis,and cell cycle of hu-manovarian cancer cells.Methods:The expression of miR -300 in the normal ovarian epithelial cell and ovarian cancer cells,and the silencing efficiency of miR-300 siRNA was detected by quantitative Real time PCR(qRT-PCR).After siRNA and NC transfected into ovarian cancer cells for 48 hours,the proliferation of ovarian cancer cells were analyzed by CCK-8,the apoptosis and cell cycle of ovarian cancer cells was analyzed by flow cytometry.Re-sults:The expression of miR-300 was significantly upregulated in ovarian cancer cells comparing with normal ovarian epithelial cells and could be effectively silenced by miR-300 siRNA.Proliferation of ovarian cancer cells were inhib-ited after transfected miR-300 siRNA,but cells apoptosis were promoted.Cell cycle of ovarian cancer cells were ar-rested at stage of G1 after transfected miR-300 siRNA.Conclusion:miR-300 play an important role on the prolifer-ation,apoptosis and cell cycle of ovarian cancer cells.