miRNA-302 c在子宫内膜癌组织中的表达及其与临床病理资料的关系

摘要

Objective:To investigated the expression of the miR-302c in tissue of endometrial cancer and its re-lationship between clinical pathological feature. Methods:Collected 49 cases tissue specimens of endometrial cancer and 39 cases of normal endometrial tissue in Shengjing Hospital of China Medical University from 2012 to 2015. The qRT-PCR was used to detect the miR-302c expression and analyse the relationship between the expression level of miR-302c and the clinical pathological data. Results:Real-time PCR was used to detect that the expression level of miR-302c,in endometrial cancer tissueit was lower than that in normal endometrial tissue,and the difference was statistically significant(P=0. 048,﹤0. 05). Moreover,the expression level of miR-302c was statistically different in endometrial cancer tissue of stageⅠand stageⅡ,Ⅲ,Ⅳ(P=0. 010,﹤0. 05). In well differentiated,moderately dif-ferentiated,poorly differentiated endometrial cancer tissue,the expression level of miR-302c is also statistically dif-ferent(P=0. 035,﹤0. 05). miR-302c expresses statistically differently in endometrial cancer tissue with or without lymph node metastasis(P=0. 008,﹤0. 05). Conclusion:The expression level of miR-302c in endometrial cancer tissue was lower than that in normal endometrial tissue,and the difference was statistically significant. The expression level of miR-302c is statistically different in different surgical-pathological stages,different degrees of histological differentiation,with or without lymph node metastasis. Conclusion there may be a certain correlation between the ex-pression of miR-302c with the occurrence of endometrial cancer,surgical-pathological stage,degrees of histological differentiation,lymph node metastasis.%目的：探讨miRNA-302c在子宫内膜癌组织中的表达及其与临床病理资料之间的关系。方法：收集中国医科大学附属盛京医院2012年-2015年子宫内膜癌患者手术标本49例及对照组正常子宫内膜组织39例，应用实时荧光定量PCR（ Real-time PCR）方法检测子宫内膜癌组织及正常子宫内膜组织中miRNA-302c的表达情况，分析miRNA-302c在子宫内膜癌组织中的表达与临床病理资料之间的关系。结果：miRNA-302c在子宫内膜癌组织中的表达水平低于正常子宫内膜组织，其差异具有统计学意义（ P＝0.048，﹤0.05）。miRNA-302c在手术分期为Ⅰ期的子宫内膜癌组织中的表达与其在Ⅱ、Ⅲ、Ⅳ期的表达进行比较，差异有统计学意义（ P＝0.010，﹤0.05）；miRNA-302c在不同组织学分化程度（高、中、低分化）的子宫内膜癌组织中的表达的差异具有统计学差异（P＝0.035，﹤0.05）；有淋巴结转移与无淋巴结转移的子宫内膜癌组织中， miRNA-302c表达水平差异具有统计学意义（ P＝0.008，﹤0.05）；而miRNA-302c在子宫内膜癌组织中的表达情况与其他临床病理资料之间无明显统计学关系。结论：miRNA-302c在子宫内膜癌组织中的表达低于正常子宫内膜组织，其差异具有统计学意义。另外，在不同手术病理分期（Ⅰ、Ⅱ、Ⅲ、Ⅳ期），不同的组织学分化程度（高、中、低分化），有、无淋巴结转移的子宫内膜癌组织中，miRNA-302c表达水平具有统计学差异。综上所述，miRNA-302c与子宫内膜癌的发生、手术分期、组织学分化程度，有无淋巴结转移存在一定的相关性。