上皮-间质转换在乳腺癌新辅助化疗耐药中的作用

摘要

目的:探讨上皮-间质转换标记物 E-cadherin和Vimentin在乳腺癌新辅助化疗耐药患者中的表达及与HER-2的关系.方法:收集我院行乳腺癌改良根治术患者75例,其中经临床和病理诊断为对新辅助化疗耐药的患者32例,所有患者均手术切除病灶并行病理检查,采用免疫组化二步法检测上皮-间质转换标记物 E-cadherin和Vimentin的表达水平.结果:乳腺癌耐药组Vimentin表达水平增加,而 E-cadherin表达下降,组间比较差异具有统计学意义(P<0.05);在43例HER-2阳性组织中,Vimentin表达水平增加,E-cad-herin降低,与HER-2阴性表达组比较差异具有统计学意义(P<0.05);进一步采用spearman相关分析显示,HER -2与 E -cadherin蛋白表达呈负相关(r= -0.336,P=0.031),而与Vimentin表达呈正相关(r=0.587,P=0.004).结论:在乳腺癌新辅助化疗耐药患者中,特别是HER-2阳性患者,可能存在上调Vimen-tin的表达、下调 E-cadherin的表达促进上皮-间质转换而促使耐药的发生.%Objective:To study the epithelial-mesenchymal transition markers of E-cadherin and Vimentin ex-pression in breast cancer with neoadjuvant chemotherapy drug resistance and its relationship with HER-2.Methods:75 cases of breast cancer patients were collected,which of 32 cases of patients were with neoadjuvant chemotherapy drug resistance diagnosed by the clinical and pathological.All patients underwent surgical removal of the lesion and were detected with immunohistochemical for E-cadherin and Vimentin expression.Results:The expression of Vimen-tin level increased in resistance group,and E-cadherin expression decreased(P<0.05).In 43 cases of HER-2 positive group,Vimentin expression level increased and E-cadherin decreased,the differences were statistically sig-nificant comparing with its HER-2 negative expression group(P<0.05).Spearman correlation analysis showed that HER-2 was negatively correlated with E-cadherin expression(r= -0.336,P=0.031),and was positively related with Vimentin expression(r=0.587,P=0.004).Conclusion:In breast cancer patients with neoadjuvant chemother-apy drug resistance,especially with HER-2 positive patients,there may be raising the expression of Vimentin,and down-regulating E-cadherin expression to promote epithelial-mesenchymal transition and prompt the occurrence of drug resistance.