PADI4在类风湿关节炎患者血液中的表达

摘要

目的 探索肽基精氨酸脱亚胺基酶4(PADI4)在早期类风湿关节炎(RA)患者血清中的表达.方法 收集早期RA(发病6月)和早期骨关节炎(OA,发病6月)患者的血清,ELISA方法检测患者血清中的PADI4、抗环瓜氨酸肽抗体(anti-CCP)和类风湿因子(RF)的水平,同时检测超过3年的RA患者血浆中这3种物质的水平.采集健康者以及系统性红斑狼疮(SLE)患者的血样进行分析,作为对照.结果 68.7％的早期RA患者的PADI4水平明显高于早期OA患者.长时间患RA患者的PADI4表达量高于SLE患者,但与健康人水平没有多大的差别.在RA患者血液中,PADI4的水平与RF和anti-CCP的水平密切相关.结论 PADI4可能在RA发病起始过程中发挥作用,PADI4的表达水平可能与疾病活动和一些临床表现有关.%Objective To investigate the expression of peptidyl arginine deaminase-4 (PADI4) in serum from patients with early rheumatoid arthritis (RA). Methods Serum of patients with early RA and early osteoarthritis (OA) were collected. The PADI4, anti-cyclic citrullinated pep- ' tide (anti-CCP) and rheumatoid factor (RF) level in the samples were detected by ELISA. The levels were also detected in RA patients with the disease duration over 3 years. Blood from health people and patients with systemic lupus erythematosus (SLE) were studied as controls. Results Levels of PADI4 were obviously higher in blood of 68.7% early RA patients when compared with samples from patients with OA. The PADI4 level was also higher in RA patients with long disease duration than that from the samples of patients with SLE, but there was no difference from the level in blood of health people. In serum of patients with RA, the PADI4 level was significantly associated with levels of RF and anti-CCP. Conclusion PADI4 may play a role in the beginning of RA, and the PADI4 level may be related with disease activity and clinical symptoms.