Objective To investigated the effect and chronergy of Xingnao Kaiqiao acupuncture with neiguan and ditch acupoint on the cerebral ischemia reperfusion in rat. Methods The improved intraluminal thread ap-proach was used to build rat model of local cerebral ischemia reperfusion. The rats were randomly divided in-to model group (MC) , acupuncture group (A), sham, and normal group. The total mortality, early mortality, Garcia JH score, blood microcirculation of the brain and cerebral ischemia area were investigated 1, 3, 5, 7 day poster-operation to evaluate the protective effect of acupuncture on cerebral ischemia reperfusion. Multi-ple factor ANOVA analysis was performed to investigate the associated factors-time and acupuncture. Results The difference in total mortality between acupuncture groups and model groups was not statistically signifi-cant (P>0.05), but was significant in early mortality (P<0.05). There were a significant differences in brain microcirculation, neurological function score and cerebral infarct volume, between model group and acupunc-ture group(P<0.01). The difference between acupuncture factor and time factor was significant(P<0.01) and it is most obvious between 3 day and 5 day after surgery. Moreover, acupuncture and time factor had an in-teractive effect on the brain microcirculation (P<0.01). Conclusion Xingnao Kaiqiao acujpuncture with nei-guan and ditch acupoint protects rats from cerebral ischemic injury, and this kind of protective effect is most obvious between the third and fifth day after reperfusion.%目的：观察针刺“醒脑开窍法”主穴内关、水沟对脑缺血再灌注模型大鼠的保护作用和作用的时效性。方法采用改良线栓法制备急性局部脑缺血再灌注模型大鼠，随机分为针刺组和模型对照组，另设假手术组和正常对照组。针刺组针刺“醒脑开窍法”主穴内关、水沟，其他各组不针刺。观察针刺1、3、5、7d的效应。用总死亡率和早期死亡率、Garcia神经功能评分、脑微循环灌注量、脑梗死体积评价针刺对缺血再灌注模型大鼠的保护作用，析因方差分析针刺因素与时间因素的效应。结果针刺组和模型对照组之间总死亡率差异没有统计学意义（P>0.05），但针刺组的早期死亡率明显低于模型对照组，其差异有统计学意义（P<0.05）；针刺组和模型对照组之间的神经功能评分、微循环灌注量和脑梗死体积的差异都有统计学意义（P<0.01），针刺因素和时间因素的效应也有统计学意义（P<0.01），在针刺3～5 d之间针刺因素的效应最明显；针刺因素与时间因素对微循环灌注量的交互作用有统计学意义（P<0.01）。结论针刺内关、水沟穴减轻和修复急性局部脑缺血再灌注模型大鼠脑损伤的作用在损伤后3～5d最明显，这种作用有显著时效性。
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