[目的]为了探索食用级牛皮脱毛方法.[方法]采用碱-酶联合方法,以碱液浓度、碱发时间、酶解时间、酶解温度、酶液 pH、酶添加量为研究因素,在单因素基础上,试验应用 Plackett-Burman 设计、响应面设计针对牛皮、牛蹄脱毛进行工艺参数优化,并建立二次回归方程.[结果]最佳脱毛工艺参数为:碱液浓度为 6%,碱发时间为23 h,碱性蛋白酶添加量为0.3%,酶解温度为 53 ℃,酶液 pH 为 10.2,酶解时间为 3 h.[结论]最佳脱毛参数条件下的牛蹄脱毛率为 92.51%,牛皮脱毛率为 94.01%,碱-酶联合脱毛的方法对于食用级脱毛具有一定应用价值.%[Objective]To explore the hair removal method of cattle leather and hoof for edible utiliza-tion.[Method]The alkali-enzyme combined procedure was designed with Plackett-Burman and response surface method.The factors affecting processing efficiency,including alkali concentration,alkali time,enzy-molysis time,enzymolysis temperature,enzyme pH and enzyme amount,were selected and optimized and two relative regression equations were established.[Result]The optimal process parameters were as fol-lows:6% of alkali solution concentration,23 hours of alkali treatment,0.3% of alkaline protease amount, 53 ℃ of enzymolysis temperature,the pH 10.2 of enzyme solution,3 hours of enzymolysis time.[Conclu-sion]The hair removal rates of hoof and leather were 92.51% and 94.01 under optimized conditions re-spectively.The alkali and enzyme combination method for edible utilization of cattle leather and hoof was promising.
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