环丙沙星在模拟水生态系统中的动态研究

摘要

利用高效液相色谱法对环丙沙星(CPFX)在微宇宙模拟水生态系统中的归趋,包括CPFX在水体、沉积物、生物体[皇冠草(Echinodorus amazonicus)、河蚬(Corbicula fluminea)、异育银鲫(Carassius auratus gibelio)]等不同分配相及生物体不同组织中的动态变化过程进行了研究.结果表明:在模拟水生态系统中加入500.00 μg·L-1 CPFX后,药物部分被水生生物所吸收,部分沉降进入底泥中.在各相中的CPFX残留量随时间不断变化,1 d内水体中药物含量降至15.30 μg·L-1,而底泥中药物含量升至385.57 μg·kg-1.CPFX不同程度地富集和残留于水生生物体中,皇冠草对CPFX的吸收量大,尤其是叶片部分1 d内含量高达795.43 μg·kg-1,随后逐步降低;河蚬、异育银鲫对CPFX有明显的吸收积累,并且在体内的残留时间可长达45 d,不同组织对CPFX的吸收积累存在明显差异,在内脏中的积累量比肌肉组织高.不同生物体中CPFX的分布和富集存在着较大差异,其趋势为皇冠草＞河蚬＞异育银鲫.%Dynamics,e.g.distribution,accumulation and transfer,of ciprofloxacin (CPFX) in water,sediments and biota in a simulated micro-cosmic aquatic system was studied with HPLC.Results show that once CPFX was released into water body,a portion of it was assimilated by aquatic organisms and a portion deposited into the sediments.Concentration of CPFX in water,sediments and biota varied with the time.The concentration of CPFX decreased rapidly in the water,from 500.00 to 15.30 μg·L-1 within one day,but increased in the sediment up to 385.57 μg·kg-1.Enrichment and residue of CPFX in aquatic organisms varied with the species.Echinodorus amazonicus adsorbed CPFX rapidly,especially in its leaves,where the concentration of CPFX reached as high as 795.43 μg·kg-1 within one day and then turned down gradually.Carassius auratus gibelio and Corbicula fluminea were found absorbing and accumulating CPFX,which could remain in their organs as residue for as long as 45 days.Significant differences existed between these organs in CPFX bioaccumulation,and higher concentration of CPFX was found in visceral tissues than in muscular tissues.Significant differences also existed between species of organisms in CPFX distribution and accumulations,showing an order of E.amazonicus＞C.fluminea＞C.auratus gibelio.