DC-SIGN在炎症性肠病肠黏膜上皮细胞表达及与肠黏膜损伤的关系

摘要

目的 探讨树突状细胞(DC)表型分子DC-SIGN在炎症性肠病(IBD)肠黏膜上皮细胞的表达及临床意义.方法 选取2006年1月至2010年6月收治的IBD患儿32例,其中克罗恩病18例,溃疡性结肠炎14例；10例正常对照儿童.免疫组织化学方法检测肠黏膜组织DC-SIGN表达；人肠上皮细胞株NCM460经LPS (50 ng/ml)刺激,Western-Blot印迹、流式细胞术分别检测肠上皮细胞DC-SIGN以及共刺激分子CD80、CD86表达,分析肠黏膜上皮细胞DC-SIGN表达与IBD及其肠黏膜损伤的关系.结果 DC-SIGN在正常对照儿童肠黏膜组织中基本不表达或低表达,而IBD患儿肠黏膜组织中表达增强,并以肠黏膜上皮细胞为主,其中克罗恩病为61.11％ (11/18),溃疡性结肠炎为50.00％ (7/14),与正常对照儿童比较,差异均有统计学意义(P＜0.05)； DC-SIGN表达与IBD患儿的疾病活动度呈正相关(r=0.475,P＜0.01).体外培养肠上皮细胞经LPS刺激后DC-SIGN表达增强,且CD80、CD86表达相应上调.结论 IBD患儿肠上皮细胞表达DC-SIGN,与细胞转分化有关；DC-SIGN可能介导肠上皮细胞在IBD及肠黏膜损伤中发挥DC样的免疫调节作用.%Objective To explore the expression of dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) on intestinal epithelial cell (IEC) in inflammation bowel disease and its possible clinical significance in the intestinal mucosal lesion. Methods A total of 32 children diagnosed with inflammation bowel disease (18 cases of Crohn's disease and 14 cases of ulcerative colitis) and 10 healthy children (control) were recruited from Jan. 2006 to Jun. 2010. The expressions of DC-SIGN, CD80 and CD86 on IECS (NCM460) with or without LPS (50ng/ml) stimulation was detected by western-blot and flow cytometry. The relationship between the expression of DC-SIGN and the mucosal lesion was analyze. Results The higher expression of DC-SIGN was observed in intestinal biopsies from the patients with inflammation bowel disease, as compared with the normal (P＜0.05). The positive rate of DC-SIGN expression, mainly occurred on IECs, is 61.11% (11/18) in crohn's disease and is 50% (7/14) in ulcerative colitis. Meanwhile the expression of DC-SIGN was positively correlated with disease activity index (r=0.475, P＜0.01). The expressions of DC-SIGN on NCM460 cells were up-regulated by LPS stimulation in vitro, and meanwhile CD80, CD86 expression was up-regulated accordingly. Conclusions This study demonstrated that the intestinal epithelial cells of children with BD were capable of expressing DC-SIGN, which may be related to cell transdifferentiation. DC-SIGN probably has an important immunomodulatory effect on the injury of intestinal mucous membrane mediated by IECs in the inflammation bowel disease.