先天性心脏病伴肺动脉高压患儿血清ghrelin的表达及意义

摘要

Objective To investigate the serum levels of ghrelin in congenital heart disease with pulmonary artery hypertension (PAH) and its significance. Methods Fifty children with congenital heart disease admitted to the hospital from May 2009 to Feb 2012 were selected. According to the pulmonary artery systolic pressure, the patients were divided into normal PAH group (21 cases), slight PAH group (17 cases) and moderate or severe PAH group (12 cases). 15 healthy children were selected as control group. The height and weight of the children in each group were measured, and serum levels of ghrelin and ET-1 were detected using double-antibody sandwich ELISA. Results In comparison to controls, serum levels of ghrelin and ET-1 significantly increased in experimental groups (P＜0.01); compared with the normal PAH group, serum levels of ghrelin and ET-1 were higher in both slight PAH group and moderate or severe PAH group (P＜0.01); the serum ghrelin levels were positively correlated with ET-1 and pulmonary artery systolic pressure in both slight PAH group and moderate or severe PAH group (r=0.577～0.840, P＜0.05). Conclusions Serum ghrelin increased in congenital heart disease with PAH may be related closely to PAH.%目的 探讨血清ghrelin在先天性心脏病(CHD)伴肺动脉高压(PAH)患儿中的表达水平及意义.方法 选择2009年5月至2012年2月住院手术治疗的CHD患儿50例,依据肺动脉收缩压分为无PAH组(21例)、轻度PAH组(17例)和中重度PAH组(12例)；另选健康儿童为对照组(15例).测量各组儿童身高和体质量并计算体质指数(BMI),双抗体夹心ELISA法测定其血清ghrelin和内皮素-1(ET-1)水平.结果 与对照组比较,各组CHD患儿的ghrelin和ET-1水平升高,差异均有统计学意义(P＜0.01)；与无PAH组比较,轻度和中重度PAH组患儿的ghrelin和ET-1水平升高,差异均有统计学意义(P＜0.01)；轻度和中重度PAH组患儿的血清ghrelin水平与血清ET-1水平和肺动脉收缩压均成正相关(r=0.577～0.840,P＜0.05).结论 Ghrelin在CHD伴PAH患儿中的表达水平增高,可能与PAH有密切关系.