两种微囊化成猪胰岛移植疗效的比较

摘要

Objective To observe and compare the functions of two typesmicroencapsulated adult pig pancreatic islets in vivo.Methods The diabetic rats induced by streptozocin were divided into four groups:control group,non-encapsulated islet transplantation group,encapsulated islet with sodium alginate membrane transplantation group and encapsulated islet with agarose membrane transplantation group.All rats received intraperitoneal of islet transplantation.Results There were no significant difference in mean level of the blood glucose before transplantation among four groups.On the 7th day after transplantation,the mean level of the blood glucose in four groups were (22.67±1.15)mmol/L，(18.58±2.66)mmol/L(12.27±1.36)mmol/L and (12.38±2.68) mmol/L,respectively.There were significant difference between two encapsulated islet groups as compared with the other two groups(P＜0.01).The survival period of two encapsulated islet transplantation groups were more longer than that of the other two group(P＜0.05 and P＜0.01) respectively.The survival period of encapsulated islet with sodium alginate membrane transplantation group was longer than that of encapsulated islet with agarose membrane transplantation group(P＜0.05).Conclusion Microencapsulated adult pig islets with sodium alginatea and agarose can survive in diabetic rats with their biological activity,but the survival period of encapsulated islet with sodium alginate membrane transplantation group was longer than that of encapsulated islet with agarose membrane transplantation group.%目的 通过对同一来源的成猪胰岛采用两种不同的免疫隔离材料进行微囊化包裹，并植入糖尿病大鼠腹腔内加以比较，以明确两种方法间的优劣。方法 将同一来源的成猪胰岛分成三组：未微囊化组、海藻酸钠微囊组和琼脂糖微囊组。24只糖尿病大鼠随机分为4组：对照组、未微囊化胰岛移植组、海藻酸钠微囊化胰岛移植组和琼脂糖微囊化胰岛移植组，分别进行腹腔内胰岛移植。结果 4组糖尿病大鼠胰岛移植前血糖水平无显著差异，移植后1周分别为(22.67±1.15)mmol/L，(18.58±2.62)mmol/L，(12.27±1.36)mmol/L和(12.38±2.68)mmol/L，后2组与前2组相比均有显著差异，且生存期明显长于前2组，但琼脂糖微囊化胰岛组的生存期明显短于海藻酸钠微囊化胰岛组(分别为平均57.10d和145.17d)。结果 两种包膜材料制备的微囊化成猪胰岛均可存活于糖尿病大鼠体内并发挥生物功能，但海藻酸钠微囊化胰岛组较琼脂糖微囊化胰岛组的存活期明显延长。