CD27和CD28在肺结核病人抗原特异性CD4~+T细胞中的表达分析

摘要

Objective To study the expression of CD27 and CD28 in antigen-specific CD4~+T cells in patients with pulmonary tuberculosis and healthy people, and understand the role of differentiated stages of CD4~+T cells in the pathogenesis of tuberculosis. Methods The expression of CD27 and CD28 was analyzed by CD4, CD154, CD27 and CD28 staining and flow cytometry. The distributions of CD27 and CD28 in antigen-specific CD4~+T cells were compared between patients with pulmonary tuberculosis and healthy controls. Results In patients of pulmonary tuberculosis, the frequencies of CD27 + CD28 + (early differentiated stage), CD27~- CD28~+ and CD27~+ CD28~- (intermediate differentiated stage), CD27~- CD28~-(fully differentiated stage) T cell subsets in antigen specific CD4~+T cells were (49. 55 ±6. 15)%, (26. 85 ±3. 87)% ,(7. 2 ± 1.37)% and ( 16. 35 ±3.97)%, respectively. In healthy controls, the frequencies of the four subsets in antigen-specific CD4~+T cells were ( 51.81 ± 4. 94 ) %, ( 29. 83 ± 5.33 ) %, ( 12. 65 ±4. 48)% and (5.7±2)%, respectively. The early differentiated CD4~+T cell was the major subset both in patients and healthy people, however, which had significant difference compared with the fully differentiated subset ( t = 2. 26, P < 0. 05 ). Conclusion The population frequency of the fully differentiated CD4~+T cells in patients with pulmonary tuberculosis was significantly higher than that in healthy people. This suggested that the differentiation degree of the antigen-specific CD4~+T cell might be related with pulmonary tuberculosis.%目的 研究肺结核病人与健康人外周血结核抗原特异性CD4~+T细胞中CD27和CD28的表达情况,了解不同分化程度T细胞在结核发病中的作用.方法 以荧光标记的抗CD4、CD154、CD27、CD28四种抗体共染色,用流式细胞仪检测肺结核病人和健康人外周血CD4~+ CD154~+ T细胞中CD27和CD28的分布比例.通过对比分析,了解不同分化阶段CD4~+T细胞在病人与健康人之间的分布差异.结果 特异性结核抗原刺激后,肺结核病人外周血CD4~+ CD154~+ T细胞中CD27~+ CD28~+(早期分化)、CD27~- CD28~+ 和CD27~+ CD28~-(中期分化)以及CD27~-CD28~-(晚期分化)T细胞的比例分别为(49.55±6.15)%、(26.85±3.87)%、(7.2±1.37)%和(16.35±3.97)%;而在健康人中这四群细胞的比例分别为(51.81 ±4.94)%、(29.83±5.33)%、(12.65±4.48)%和(5.7±2)%.结核病人与健康人以早期分化CD4~+T细胞为主,在晚期分化CD4~+T细胞比例上差异有统计学意义(t=2.26,P<0.05).结论 肺结核病人抗原特异性CD4~+T细胞中晚期分化阶段细胞的比例显著高于健康人,提示抗原特异性CD4~+T细胞的分化程度与结核病相关.