NF-κB在大鼠实验性大脑内出血脑组织表达的研究

摘要

Objective To investigate the expression of nuclear factor-KB( NF-κB ) in brain tissues at different time after experimental intercerehral hemorrhage ( ICH ) in rats. Methods To utilize stereotactic apparatus and infuse autologous blood into the left caudate nucleus to build up rat experimental ICH model. The expression of NF-κB in cerebral tissues were detected by immunohistochemistry techniques. Results The expression of NF-κB was not only in neurons hut also in glial cells and the endfoot of astrocytes. NF-κB was upgraded at 12 h and peaked at 2 d, then decreased. Conclusion NF-κB may have a effect on the formation of edema after ICH.%目的 研究大鼠实验性大脑内出血后脑组织核转录因子κB(NF-κB)的表达及变化规律.方法 采用立体定向技术将自体不凝血注入大鼠尾状核区制备不同时间段的大脑内出血模型,用免疫组化染色法检测不同出血时间脑组织NF-κB的表达.结果 NF-κB在神经元、神经胶质细胞及星形胶质细胞足突广泛表达.表达的阳性细胞数在出血后12 h上升,2 d达高峰,之后下降.结论 NF-κB可能参与了出血后脑水肿的形成.