2D-DIGE技术在蛋白质组学中的应用

摘要

双向荧光差异凝胶电泳(2D-DIGE)作为一种新型的蛋白质组分析技术,已经被广泛应用于动物、植物、微生物以及人类差异蛋白的研究.在动物医学方面,采用DIGE技术,通过对不同类型,不同个体的细胞、组织、或经过不同处理和不同生长条件下蛋白质表达差异分析,在研究疾病的分子机理、分子诊断、药物作用机理、毒理学等方面都有广泛的应用.在植物学方面,该技术可以用来分离和分析植物亚细胞结构蛋白质组以及在生物和非生物胁迫下,植物细胞中蛋白质表达的差异性,从而建立差异蛋白相互作用网络图,从而为研究伤害机制提供一定的依据.%Two-way fluorescence difference gel electrophoresis (2D-DIGE) as one of new analytical techniques was widely used in study of animals , plants , microbes and proteins of human differences. In animal medicine , using DIGE technology , through different types of cells in different individuals, organizations, or through different treatments and different protein expression under different growth conditions analyzed in the study of molecular mechanisms of disease, molecular diagnostics, drug mechanism, drug science has a wide range of application areas. In botany , the technology can be used to isolate and analyze the subcellular structures of plant proteins as well as in biotic and abiotic stress, plant cells differences in protein expression in order to establish differences in protein interaction network graph, and thus to study the damage mechanism to provide some basis.