壳聚糖-Zn(II)亲和层析介质的制备及表征

摘要

以自制的壳聚糖微球为载体，环氧氯丙烷（ ECH）为活化剂，亚氨基二乙酸（ IDA）为螯合配基，Zn2＋为螯合金属离子制备壳聚糖－Zn（ II）亲和层析介质。最佳活化工艺：M壳聚糖（ g）∶VECH （ mL）为1∶4、NaOH浓度为1．2 mol／L、活化温度为50℃，活化时间为4 h，测得环氧基修饰密度达0．2472 mmol／g；最佳螯合工艺：IDA作为配基、浓度为0．6 mol／L、反应温度为70℃、反应时间为6 h，ZnCl2作为螯合金属盐、浓度为0．1 mol／L、反应时间为3 h，Zn2＋螯合量达到最大值。通过红外光谱表征，证明壳聚糖与Zn（ II）发生了螯合配位反应，生成了壳聚糖－Zn（ II）配合物。%The chitosan-zinc( II) affinity chromatography medium chelates with Zn 2+using self-made chitosan microspheres as sub-strate.The iminodiacetic acid(IDA)was attached onto chitosan microspheres activated by epichlorohydrin (ECH).The optimal epoxy density above 0.2472 mmol/g gel was achieved at the proportion of epoxy chloropropane and chitosan microspheres for 1∶4 in the solu-tion consisting of 1.2 mol/LNaOH at 60℃for 6 h.The study on linkaging of IDA , as ligands , demonstrated that the supporter was synthesized in the solution with 0.6 mol/L IDA at 70℃for 6 h.When chelating of ZnCl 2 , as chelating metal salts , synthesized in the solution with 0.1 mol/L ZnCl2 for 3 h, the adsorption of Zn2+was up to the maximum.Infrared by IR spectra, the chelating ligand re-action of chitosan and zinc ( II) and the generation of the chitosan-zinc ( II) complexes were achieved .