为了探索人脐带血血红蛋白的纯化方法，用0．9％生理盐水洗涤脐带血，经蒸馏水进行粗提，经Q300阴离子交换纯化血红蛋白，对纯化后的血红蛋白进行浓缩，得到终产品。测定血红蛋白浓度及高铁血红蛋白含量，并用SDS－PAGE和HPLC进行纯度分析，通过血样分析仪进行氧饱和曲线分析。纯化和浓缩后血红蛋白浓度为11．5 g／dl，高铁血红蛋白含量≤1％；经SDS－PAGE和HPLC分析血红蛋白纯度较高，不含杂蛋白，凝胶成像系统分析纯度为99．10％±0．70％；纯化前后血红蛋白的氧合曲线无明显偏移，不影响其载氧活性。研究证明，成功地得到了人脐带血血红蛋白纯化的方法。%The aim of the study is to establish the purification methods of placenta hemoglobin .The placenta hemoglobin was eluted by 0.9%Na chloride(NS) and extracted by distilled water, which was further purified by Q300 anion-exchange chromatography, and then the final products was got by concentrating the purified hemoglobin .The concentration of hemoglobin and the content of methemo-globin of the final products was measured , and the purity was analyzed by SDS-PAGE and HPLC,the oxygen saturation curve was ana-lyzed through the blood analyzer .The concentration of final products was 11.5 g/dl,and the content of methemoglobin is 1% or less;hemoglobin had a higher purity by the analysis of SDS-PAGE and HPLC, excluding mixed protein , the purity was 99.10%±0.70%analyzed by Gel Imaging System;the oxygenation of hemoglobin curve had no significant deviation after purification , the activity of car-rying oxygen would not be affected .The research shows that the methods to purify placenta hemoglobin were established .
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