基于高通量测序技术对沙湖水体细菌多样性的分析

摘要

In order to investigate the structure and diversity of microbial community in Shahu Lake,the total DNA of bacteria from wa-ter samples was extracted by E.Z.N.A. Water DNA extraction kit, then the V4-V5 region of 16S rRNA gene was sequenced by MiSeq technology.Mothur software and R language were used to analyze the microbial community structure and diversity.A total of 427975 optimized sequences were obtained,and 20 Phylum,50 Class,95 Order,161 Family, and 276 Genus were predicted at the 97% similarity level.The OTUs for A,B,C and D were 497,496,487 and 511,respectively.Proteobacteria,Cyanobacteria,Acti-nobacteria and Bacteroidetes are dominant groups.The microbial community structure in Shahu Lake is complex,but the composition and diversity of microbial community structure are not significant at different sites.%为了解沙湖水体中细菌群落结构和多样性,采用E.Z.N.A. 水样DNA提取试剂盒提取水样总DNA,对细菌群落的16S rRNA基因的V4~V5区进行MiSeq测序,利用Mothur软件和R语言进行细菌群落结构及多样性的分析.共得到427975条优化序列,在97%的相似水平下对OTU进行统计分析,得到20门,50纲,95目,161科,276属.样点A、B、C、D的OTU分别为497、496、487和511.优势类群为变形菌门(Proteobacteria)、蓝细菌门(Cya-nobacteria)、放线菌(Actinobacteria)、拟杆菌(Bacteroidetes).沙湖水体中细菌群落结构复杂,但不同位点细菌群落结构的组成以及多样性差异不显著.