Immobilized-metal affinity chromatography adsorbent with paramagnetism and its application in purification of the proteins in the natural products

摘要

Aim To investigate a new method for synthesis of an Immobilized-Metal Affinity Chromatography(IMAC) adsorbent with superparamagnetism(Fe3O4/SiO2-GPTMS-Asp-Co).Method The magnetic microspheres were synthesized in situ modification and the transmission electron microscope,field emission scanning electron microscopy,X-ray diffractometer,energy spectrometer and vibrating sample magnetometer were used to characterize the appearance,particle size distribution,phase composition,chemical constituents and magnetic properties of silica magnetic microspheres(MMS).Results The silica MMS prepared by reverse microemulsions exhibit a superior core-shell structure.The size distribution of the microspheres varies from between 100 nm to 200 nm and the main phase of microspheres is amorphous SiO2 and spinel Fe3O4.The microspheres retain superparamagnetism and can be used as biomaterials.Conclusion The result indicates that the IMAC adsorbent we prepared has outstanding advantages in the separation of the natural products proteins from the crude bacterial lysate,such as simple operation,high selectivity and capacity.