肝素钠抗凝血浆测定电解质浓度及正常参考值研究

摘要

目的 比较用间接电极法测定血清和肝素钠抗凝血浆电解质浓度的差别,探讨肝素钠抗凝血浆电解质浓度的正常参考值.方法 采用罗氏MUDULAR ISE900模块式电解质分析仪检测70例体检者血清和肝素钠抗凝血浆的电解质浓度.结果 血清与肝素抗凝血浆的K+、Na+、Cl-的测定结果差异有统计学意义(P＜0.01),平均差值分别为0.24、1.42、0.94 mmol/L,相对偏差分别为5.60%、0.99%和0.89%.Na+和Cl-差别小,相对偏差小于1%,在临床上无实际意义;K+的差异已超过5%,且相对恒定.统计1 739例健康体检者肝素钠抗凝血浆电解质浓度,以x±2s为正常参考质范围,则K+:3.27~4.67 mmol/L,Na+:137.9~147.5 mmol/L,Cl-:99.2~110.8 mmol/L.结论 肝素钠是一种较理想的电解质抗凝剂,适合于电解质的测定,但是肝素钠抗凝血浆K+明显低于血清,不能沿用血清的正常参考值,建议各实验室建立自已的正常参考范围.%Objective To compare the; difference of serum and heparin sodium anticoagulation plasma electrolyte concentration by indirect electrode method,and to explore the normal reference values of heparin sodium anticoagulation plasma electrolyte con -cent ration .Methods Using the Roche MUDULAR ISE900 modular electrolyte analyzer to test 70 healthy persons electrolyte in serum and heparin sodium anticoagulant plasma . Results The difference of K,Na and Cl lenels were statistically significant (P< 0 .01),The average difference of the serum and plasma K,Na and Cl were 0 .24 mmol/L,1 .42 mmol/L and 0 .94 mmol/L respectively,and the relative deviation were 5 .6%,0 .99% and 0 .89%.The difference of Na and Cl was small and the relative deviation were less than l%,which no practical significance was in clinic ;while the difference of the K in serum and plasma has exceeded 5/o and constant relatively .We analyzed the concentration of plasma electrolyte in 1 739 healthy examination adults .The normal reference value was le±2s,then K+ (3 .27-4 .67)mmol/L,Na+ (137 .9-147 .5)mmol/L,C1" (99 .2-110 .8)mmol/L .Conclusion Heparin was an ideal anticoagulant,which was suitable for the determination of electrolyte .However,the plasma K was significantly lower than that in serum,which have been unable to use the normal reference values of serum .Every laboratory should establish their own normal reference range .