基因组编辑技术在植物基因功能鉴定及作物育种中的应用

摘要

Plant genome can be modifiedvia current biotechnology with high specificity and excellent efficiency. Zinc finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and clustered regularly inter-spaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system are the key engineered nucleases used in the genome editing. Genome editing techniques enable gene targeted mutagenesis, gene knock-out, gene in-sertion or replacement at the target sites during the endogenous DNA repair process, including non-homologous end joining (NHEJ) and homologous recombination (HR), triggered by the induction of DNA double-strand break (DSB).Genome editing has been successfully applied in the genome modification of diverse plant species, such asAra-bidopsis thaliana,Oryza sativa, andNicotiana tabacum. In this review, we summarize the application of genome editing in identification of plant gene function and crop breeding. Moreover, we also discuss the improving points of genome editing in crop precision genetic improvement for further study.%利用生物技术可以对植物基因组进行高效、精准、特异的修饰.锌指核酸酶(Zinc finger nucleases,ZFN)、转录激活样效应因子核酸酶(Transcription activator-like effector nucleases,TALEN)、成簇规律间隔短回文重复序列(Clustered regularly interspaced short palindromic repeats,CRISPR)/Cas9(CRISPR-associated 9)是目前基因组编辑技术应用中的关键工程核酸酶.通过产生DNA双链断裂(Double-strand breaks,DSBs)激活植物内源修复途径(包括非同源粘性末端连接和同源重组修复),基因组编辑技术可以实现对靶位点的定点突变、缺失或者基因的插入与替换.基因组编辑已经被广泛地应用到各种植物的基因组修饰中,如拟南芥、水稻、烟草等.本文主要概述了基因组编辑技术在植物基因功能鉴定及作物遗传育种中的应用,并对其未来在作物精准改良中需要完善的相关问题进行了探讨.