鸡胚免疫器官发育中肥大细胞的观察

摘要

为了探讨肥大细胞(MC)在鸡胚免疫器官中的作用,并提供组织形态学依据,对不同日龄鸡胚胸腺、法氏囊、脾脏采用Carony's液固定,阿尔新兰染色-藏红O复染(alcian blue-safrianin O,AB/SO)并对其肥大细胞(Mast cell,MC)的形态、分布及数量变化进行观察.结果表明,Carnoy's液固定,AB/SO染色可以清晰地显示MC的组织结构,MC呈圆形、椭圆形和不规则形,大小不一;胸腺中MC主要分布在髓质,并有少量分布于血管和小叶间结缔组织;法氏囊中MC在淋巴小结周围的组织中分布;脾脏中MC分布在淋巴小结周围,血窦、血管中偶见.18日龄前脾脏、19日龄前法氏囊、20日龄前胸腺中MC的数量与器官发育呈正相关,其后MC的数量下降是否与MC的排出有关尚不清楚.%The morphology, distribution and quantity change of thymus, bursa and spleen of chick embryos with different day-old were observed by Carony's fixation and AB/SO to study the effect of MC on immune organs in chick embryos and provide the morphological basis. The results show that the shape of MC is oval, ovoid and irregular, most MC in the thymus distributes in medulla and a few MC in the thymus distribute in blood vessel and inter lobular connective tissue, MC in the bursa distribute the tissue around lymph nodule, MC in the spleen mainly distribute around lymph nodule, there are positive correlations between organ development and MC quantity in the spleen before 18 day-old, in the bursa before 19 day-old and in the thymus before 20 day-old. The correlation between MC quantity descent and MC elimination should be further studied.