黑木耳粉对染矽尘大鼠肺纤维化的影响

摘要

To study the effect of auricularia auricular powder (AAP) on pulmonary fibrosis to find an effective treatment on early-stage salvia . Silicosis models were established by direct tracheal instillation of silica 200 mg/kg into rat lungs non-surgically. After 24 hours,rats were given AAP by feeding. At 14th,28th and 56th day, eight rats were killed in a group at a time. Ⅰfibrosis content , Ⅲ fibrosis content, the superoxide dismutase (SOD)activities,maleic dialdehyde (MDA) content and glutathione peroxidase (NO) content in the serum were assayed. At 14th,28th and 56th day,ⅠandⅢfibrosis content of AAP-treated groups were lower than those of contemporaneous SiO2 control group , and Ⅰand Ⅲ fibrosis content were significantly difference between the AAP-treated groupⅢand SiO2 control group (P<0.05).SOD activities were higher than those of contemporaneous SiO2 control group,and MDA content were lower than those of contemporaneous SiO2 control group, moreover,at 14 th day, SOD activities of AAP-treated groups were significantly higher than those of SiO2 control group (P <0.05). serum NO content of AAP -treated groups were lower than those of contemporaneous SiO2 control group,and serum NO content were significantly difference between the AAP-treated groupⅢand SiO2 control group(P<0.05). AAP can resist the oxidative damage and inhibit synthesization of lung collagen fibrosis to prevention silicotic fibrosis.%　　观察黑木耳粉对染矽尘大鼠肺组织纤维化的影响，探讨黑木耳粉对矽肺早期的防治作用。采用非暴露气管插管法给大鼠气管注入二氧化硅（SiO2）粉尘200 mg/kg，染尘后24 h开始以喂饲途径给予黑木耳粉，分别在14、28、56 d时分批处死动物。检测肺组织Ⅰ型胶原纤维、Ⅲ型胶原纤维、超氧化物歧化酶（SOD）、丙二醛（MDA）和血清一氧化氮（NO）含量变化。结果表明：14、28、56 d时，黑木耳粉治疗组大鼠肺组织Ⅰ和Ⅲ型胶原纤维含量与同期SiO2对照组比较有所降低，其中黑木耳粉高剂量组大鼠肺组织Ⅰ和Ⅲ型胶原纤维含量与同期SiO2对照组比较，差异均有显著性（P<0.05）；黑木耳粉治疗组大鼠肺组织SOD活性高于同期SiO2对照组，MDA含量低于同期SiO2对照组，且在14 d时，黑木耳粉各个剂量组SOD活性与SiO2对照组比较，差异均有显著性（P<0.05）；黑木耳粉治疗组大鼠血清NO含量均低于同期SiO2对照组，黑木耳粉高剂量组与同期SiO2对照组比较差异有显著性（P<0.05）。黑木耳粉能够减轻染石英尘大鼠肺组织氧化损伤，可降低肺组织胶原纤维含量，提示其对矽肺纤维化具有一定的防治作用。