响应面法优化桑葚花色苷提取工艺的研究

摘要

Studied the optimization of melberry anthocyanin extraction by the response surface methodology (RSM).On the basis of one-factor test ,the signigicance and interaction of various factors were analyzed according to the Design-Exper8.0 experimental design with 3-factors and 3-levels.The optimum conditions of melberry anthocyanin extraction were as follow:the extraction temperature 62 ℃, the extraction duration 135 min, the solid-liquid ratio 1∶25.At this condition,the extraction yield of mullberry anthcyanins was 5.09 mg/g.%利用响应面分析法（RSM）对桑椹花色苷的提取工艺进行优化。在单因素试验基础上选取试验因素与水平，根据中心组合（Box-Behnken）试验设计原理采用三因素三水平的响应面分析法对各个因素的显著性和交互作用进行分析，结果表明：桑葚花色苷提取的最佳工艺条件为：料液比1∶25，提取时间135 min，提取温度62℃，在此条件下，花色苷提取率最高达到5.09 mg/g。