六盘水野生蕨菜多糖的超声波辅助提取及含量测定

摘要

以六盘水野生蕨菜为材料，通过单因素设计以及正交试验对蕨菜多糖超声波提取的最佳工艺进行探索，并采用苯酚硫酸法对六盘水野生蕨菜多糖的含量进行测定。结果表明：蕨菜多糖的超声波辅助提取最佳工艺为料液比1∶100（g/mL）、功率70%（420 W）、时间50 min、温度35℃；蕨菜多糖的含量为245.75167 mg/g。为六盘水野生蕨菜的进一步开发利用提供理论参考。%Liu Panshui wild Pteridium revolutum as materials, the best technology of single factor and orthogonal experiment design of polysaccharides in Pteridium revolutum by ultrasonic-assisted extraction , and by phenol sulfuric acid method on the content of polysaccharide was determined in Liu Panshui wild Pteridium revolutum. The result showed that:the optimum ultrasonic-assisted extraction of polysaccharide in Pteridium revolutum as solid-liquid ratio was 1 ∶ 100 (g/mL), power of 70 %(420 W), extraction time was 50 min, temperature 35 ℃; content of polysaccharide in Pteridium revolutum was 245.751 67 mg/g. To provide theoreticalbasis for further development and utilization of Liu Panshui wild Pteridium revolutum.