研究一种含D-松醇功能成分的混合菌种发酵饮料对STZ诱导2型糖尿病大鼠肝损伤的干预作用.通过酿酒酵母和乳酸菌混合发酵含D-松醇的角豆粉(carob powder)提取液,制备一款含D-松醇功能成分的发酵型低糖饮料.采用高脂高糖饲料喂养SD大鼠4周后腹腔注射STZ建立2型糖尿病模型,将实验大鼠分为正常对照组、模型对照组、阳性对照组、发酵饮料低剂量组、发酵饮料高剂量组.灌胃发酵饮料4周后,测定大鼠空腹血糖(FBG)、肝脏指数、天门冬氨酸氨基转移酶(AST)、血清中丙氨酸氨基转移酶(ALT)含量,同时在光镜下观察各组大鼠肝组织细胞病理形态学改变.结果表明:与正常对照组相比,模型对照组大鼠血清中AST、ALT水平极显著性升高(p<0.01),HE染色显示模型对照组大鼠较正常对照组大鼠肝细胞脂肪变性明显,肝组织结构不清楚,肝细胞排列紊乱.与模型对照组大鼠相比,阳性对照组大鼠及发酵饮料剂量组大鼠血清中AST、ALT水平显著性降低(p<0.05),HE染色显示阳性对照组和发酵饮料剂量组大鼠较模型对照组大鼠肝细胞脂肪变性明显减少,肝细胞排列基本规则.结论:此含D-松醇发酵饮料对2型糖尿病大鼠的肝损伤有一定的干预作用.%This experiment studied the intervention effect of a fermented beverage enriched D-pinitol on liver damage of STZ-induced type 2 diabetic rat. To develop a fermented beverage which was low sugar, the fermen-tation of Saccharomyces cerevisiae and lactic acid bacteria with the carob powder extract enriched D-pinitol was been stuied. The type 2 diabetic rat were fed enrich-food for 4 weeks before administrated STZ, the SD rats were divided into normal control group, model control group, positive control group, low dose of fermented beverage enriched D-pinitol, high dose of fermented beverage enriched D-pinitol. After 4 weeks of a daily dose of the fermented beverage enriched D-pinitol, some biochemical parameters were measured, which includes FBG, the liver index, ALT, AST test and the pathology morphology change of rat liver tissue cell under research mi-croscope. The Results showed that:Compared with normal control group, the serum AST, ALT level increased extremely significantly (p<0.01), HE staining showed hepatocyte steatosis significantly in model control group rat, liver tissue structure was not clear, the arrangement of liver cell was disordered. Compared with model con-trol group, the serum AST, ALT level decreased significantly (p<0.05), HE staining showed liver steatosis de-creased significantly in positive control group and dose group, the arrangement of liver cell was well-regulated. The fermented beverage enriched D-pinitol has the positive intervention effect on liver damage of type 2 diabet-ic rat.
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