为探究不同质量浓度的低聚木糖调控鲤鱼免疫力及生长性能的机理，以450尾平均体质量（4．93±0．03）g的健康鲤鱼为对象，研究饲料中添加低聚木糖对鲤鱼生长性能和免疫指标的影响。以不加低聚木糖的基础饲料为对照组，试验组为分别在基础饲料中添加0．1％低聚木糖组、0．2％低聚木糖组、0．4％低聚木糖组和0．6％低聚木糖组。试验结果表明，低聚木糖可提高鲤鱼血清溶菌酶活性，但对补体旁路途径溶血活性无影响，0．6％低聚木糖组的血清溶菌酶活性显著高于对照组（P＜0．05）。对于头肾的5种免疫相关基因，低聚木糖可上调白细胞介素-1β和白细胞介素-10的基因表达，下调诱导型一氧化氮合酶和肿瘤坏死因子-α的基因表达，而对转化生长因子-β的基因表达无影响。与对照组相比，0．2％低聚木糖组、0．6％低聚木糖组的白细胞介素-1β与0．2％低聚木糖组的白细胞介素-10表达量均显著升高，所有试验组的诱导型一氧化氮合酶和肿瘤坏死因子-α的表达量均显著下降（P＜0．05）。生长性能试验结果显示，试验组鲤鱼的生长性能指标均高于对照组，其中0．6％低聚木糖组与对照组差异显著（P＜0．05）。%The effects of dietary xylo-oligosaccharides (XOS) on growth performance and immune function were investigated in 450 juvenile common carp (Cyprinus carpio) with an average initial body weight of (4 .93 ± 0 .03) g .The juveniles were randomly divided into five groups and fed basal diet ( as a control group ,CON) ,and the diet containing XOS at a rate of 0 .1% (group Ⅰ ) ,0 .2% (group Ⅱ ) ,0 .4%(group Ⅲ) ,or 0 .6% (group Ⅳ) .The results showed that there was significantly higher lysozyme activity in group Ⅳcompared with the control (P<0 .05) ,but no significant difference in alternative pathway of complement (ACH50 ) was observed in the treatment groups (P> 0 .05) .The expressions of iNOS and TNF-α were lower in the treatment groups than those in control group ,while the expressions of IL-1βand IL-10 genes were found to be up-regulated significantly (P<0 .05) ,without change in expression of TGF-βgene compared with the control group .The juveniles fed the diets containing XOS showed better growth performance than the fish fed the basal diet did ,with significant difference only between group Ⅳand the control group (P<0 .05) .The findings suggest that supplementation of XOS positively affect growth per-formance and immunity in common carp .
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