肺结核和肺外结核患者外周血淋巴细胞亚群的表达及临床意义

摘要

目的 分析肺结核和肺外结核患者的免疫特点及差异,以指导临床治疗.方法 采用流式细胞术(FCM)检测40例肺结核(肺结核组),33例肺外结核(肺外结核组)及30例健康体检(对照组)外周血CD3+细胞、CD3+ CD4+细胞、CD3+ CD8+细胞、NK细胞、B细胞的表达及CD3+ CD4 +/CD3+ CD8+比值,并进行比较分析.结果 肺结核组、肺外结核组CD3+、CD4+表达率与对照组对比差异有统计学意义(P＜0.01)；CD3+ CD4 +/CD3+ CD8+比值、CD8+表达率与对照组比较差异无统计学意义(P＞0.05).肺外结核组NK细胞、B细胞表达率与肺结核组比较差异有统计学意义(P＜0.05).结论 肺结核和肺外结核患者均伴有细胞免疫功能紊乱,外周血淋巴细胞亚群水平评估有助于了解细胞免疫状态,可为临床免疫干预提供理论依据.%Objective To analyze the immune characteristics and differences between the pulmonary tuberculosis and extrapulmonary tuberculosis so as to provide reference to clinical treatment. Methods The expression of CD3+ cells, CD3+CD4+ cells, CD3 + CD8 + cells, NK cells and B cells of peripheral blood, and percentages of CD3 + CD4 + /CD3+ CD8 + in 40 patients with tuberculosis (TB group) , 33 patients with extrapulmonary tuberculosis (extrapulmonary tuberculosis group) and 30 healthy persons (control group) were detected by flow cytometry (FCM) and the clinical data were compared. Results There were statistically significant (P0.05). The differences of expression rates in NK cells and B cells between TB group and extrapulmonary tuberculosis group were statistically significant (P<0.05). Conclusion Patients with tuberculosis or extrapulmonary tuberculosis has cellular immune function disorder, and peripheral-blood lymphocyte subsets evaluation can help clinicians to learn about the cellular immunity which can provide theoretical basis for clinical immunologic intervention.